Data analysis for 16S microbial profiling from different benchtop sequencing platforms

Pylro, V. S., Roesch, L. F. W., Morais, D. K., Clark, IanORCID logo, Hirsch, PennyORCID logo and Totola, M. R. (2014) Data analysis for 16S microbial profiling from different benchtop sequencing platforms. Journal of Microbiological Methods, 107 (Decemb). pp. 30-37. 10.1016/j.mimet.2014.08.018
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Progress in microbial ecology is confounded by problems when evaluating results from different sequencing methodologies. Contrary to existing expectations, here we demonstrate that the same biological conclusion is reached using different NGS technologies when stringent sequence quality filtering and accurate clustering algorithms are applied.
Item Type Article
Open Access Gold
Keywords Next generation sequencing, Microbial community analysis, Amplicons, Alpha diversity, Beta diversity
Project Delivering Sustainable Systems (SS) [ISPG], Optimisation of nutrients in soil-plant systems: How can we control nitrogen cycling in soil?
Date Deposited 05 Dec 2025 09:50
Last Modified 19 Dec 2025 14:35

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