A - Papers appearing in refereed journals
Delmont, T. O., Robe, P., Clark, I. M., Simonet, P. and Vogel, T. M. 2011. Metagenomic comparison of direct and indirect soil DNA extraction approaches. Journal of Microbiological Methods. 86 (3), pp. 397-400. https://doi.org/10.1016/j.mimet.2011.06.013
Authors | Delmont, T. O., Robe, P., Clark, I. M., Simonet, P. and Vogel, T. M. |
---|---|
Abstract | Full pyrosequencing runs of both direct-extracted (high yield, low DNA size) and indirect-extracted DNA (low yield, high DNA size) from the same prairie soil show that the sequence distribution of the majority of the metabolic functions and species detected were statistically similar. Although some microbial functions differed at the 95% confidence interval in bootstrap analyses, the overall functional diversity was the same. |
Keywords | soil metagenomics; DNA extraction; Nycodenz |
Year of Publication | 2011 |
Journal | Journal of Microbiological Methods |
Journal citation | 86 (3), pp. 397-400 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.mimet.2011.06.013 |
Open access | Published as non-open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | SEF |
Soil microbial community function and diversity | |
Publisher's version | Copyright license Publisher copyright |
Output status | Published |
Publication dates | |
Online | 25 Jun 2011 |
Publication process dates | |
Accepted | 18 Jun 2011 |
ISSN | 0167-7012 |
Publisher | Elsevier Science Bv |
Permalink - https://repository.rothamsted.ac.uk/item/8q8zy/metagenomic-comparison-of-direct-and-indirect-soil-dna-extraction-approaches
Publisher's version