Metagenomic comparison of direct and indirect soil DNA extraction approaches

A - Papers appearing in refereed journals

Delmont, T. O., Robe, P., Clark, I. M., Simonet, P. and Vogel, T. M. 2011. Metagenomic comparison of direct and indirect soil DNA extraction approaches. Journal of Microbiological Methods. 86 (3), pp. 397-400.

AuthorsDelmont, T. O., Robe, P., Clark, I. M., Simonet, P. and Vogel, T. M.
Abstract

Full pyrosequencing runs of both direct-extracted (high yield, low DNA size) and indirect-extracted DNA (low yield, high DNA size) from the same prairie soil show that the sequence distribution of the majority of the metabolic functions and species detected were statistically similar. Although some microbial functions differed at the 95% confidence interval in bootstrap analyses, the overall functional diversity was the same.

Keywordssoil metagenomics; DNA extraction; Nycodenz
Year of Publication2011
JournalJournal of Microbiological Methods
Journal citation86 (3), pp. 397-400
Digital Object Identifier (DOI)doi:10.1016/j.mimet.2011.06.013
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeSEF
Soil microbial community function and diversity
Publisher's version
Copyright license
Publisher copyright
Output statusPublished
Publication dates
Online25 Jun 2011
Publication process dates
Accepted18 Jun 2011
ISSN0167-7012
PublisherElsevier Science Bv

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