Novel European free-living, non-diazotrophic Bradyrhizobium isolates from contrasting soils that lack nodulation and nitrogen fixation genes - a genome comparison

A - Papers appearing in refereed journals

Jones, F. P., Clark, I. M., King, R., Shaw, L. J., Woodward, M. J. and Hirsch, P. R. 2016. Novel European free-living, non-diazotrophic Bradyrhizobium isolates from contrasting soils that lack nodulation and nitrogen fixation genes - a genome comparison. Scientific Reports. 6, p. 25858.

AuthorsJones, F. P., Clark, I. M., King, R., Shaw, L. J., Woodward, M. J. and Hirsch, P. R.
Abstract

The slow-growing genus Bradyrhizobium is biologically important in soils, with different representatives found to perform a range of biochemical functions including photosynthesis, induction of root nodules and symbiotic nitrogen fixation and denitrification. Consequently, the role of the genus in soil ecology and biogeochemical transformations is of agricultural and environmental significance. Some isolates of Bradyrhizobium have been shown to be non-symbiotic and do not possess the ability to form nodules. Here we present the genome and gene annotations of two such free-living Bradyrhizobium isolates, named G22 and BF49, from soils with differing long-term management regimes (grassland and bare fallow respectively) in addition to carbon metabolism analysis. These Bradyrhizobium isolates are the first to be isolated and sequenced from European soil and are the first free-living Bradyrhizobium isolates, lacking both nodulation and nitrogen fixation genes, to have their genomes sequenced and assembled from cultured samples. The G22 and BF49 genomes are distinctly different with respect to size and number of genes; the grassland isolate also contains a plasmid. There are also a number of functional differences between these isolates and other published genomes, suggesting that this ubiquitous genus is extremely heterogeneous and has roles within the community not including symbiotic nitrogen fixation.

Year of Publication2016
JournalScientific Reports
Journal citation6, p. 25858
Digital Object Identifier (DOI)doi:10.1038/srep25858
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Lawes Agricultural Trust
Funder project or codeDelivering Sustainable Systems (SS) [ISPG]
LAT studentship:The microbiology of lean and obese soil
Optimisation of nutrients in soil-plant systems: How can we control nitrogen cycling in soil?
Publisher's version
Copyright license
CC BY
Output statusPublished
Publication dates
Online10 May 2016
Publication process dates
Accepted25 Apr 2016
PublisherSpringer Nature
Nature Publishing Group
ISSN2045-2322

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