Changes in the frequency of the G137D and W251S mutations in the carboxylesterase E3 gene of Cochliomyia hominivorax (Diptera: Calliphoridae) populations from Uruguay

A - Papers appearing in refereed journals

Assis De Carvalho, R., Gomez Limia, C. E., Bass, C. and Lima De Azeredo-Espin, A. M. 2010. Changes in the frequency of the G137D and W251S mutations in the carboxylesterase E3 gene of Cochliomyia hominivorax (Diptera: Calliphoridae) populations from Uruguay. Veterinary Parasitology. 170 (3-4), pp. 297-301.

AuthorsAssis De Carvalho, R., Gomez Limia, C. E., Bass, C. and Lima De Azeredo-Espin, A. M.
Abstract

The New World Screwworm (NWS) fly Cochliomyia hominivorax is one of most important myiasis-causing flies in the Neotropics. It is responsible for severe losses to the livestock industry through both mortality and the loss of productivity of infested animals. In Uruguay, NWS represents a significant problem. To date this pest has been controlled by the application of chemical insecticides, mainly the pyrethroid and organophosphate (OP) classes. However, the intensive use of these compounds over many years has led to the evolution of resistance which has the potential to compromise the effectiveness of current control strategies. One mechanism by which resistance has occurred in this and related dipteran species is through two mutations (G137D and W251S) in the carboxylesterase E3 enzyme that have enhanced ability to hydrolyze certain insecticides. In this study changes in the frequency of these mutations in C. hominivorax was investigated in three different Uruguayan regions in 2003 and 2009. All three regions analyzed showed a reduction in the frequency of the G137D mutation and a significant increase in frequency of the W251S mutation, and this may be related to the current intense use of dimethyl-OP and pyrethroid insecticides. The findings of this study provide current information on the frequency of these resistance-associated mutations in NWS in Uruguay and may help select appropriate chemicals for NWS control as part of potential pest management strategies.

KeywordsScrewworm; Myiasis; Organophosphate; Pyrethroid; Resistance
Year of Publication2010
JournalVeterinary Parasitology
Journal citation170 (3-4), pp. 297-301
Digital Object Identifier (DOI)doi:10.1016/j.vetpar.2010.02.029
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
BBSRC Institute Career Path Fellowship: A genomic approach to understanding insecticide resistance in crop pests
Output statusPublished
Publication dates
Online26 Feb 2010
Publication process dates
Accepted19 Feb 2010
PublisherElsevier Science Bv
ISSN0304-4017

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