Doublesex homolog is sex-specifically spliced and governs the sexual differentiation process in the whitefly Bemisia tabaci AsiaII-1

A - Papers appearing in refereed journals

Brar, G. S., Singh, S., Shukla, J. N., Kumar, V. J., Davies, T. G. E., Kaur, G., Pandher, S and Kaur, R. 2022. Doublesex homolog is sex-specifically spliced and governs the sexual differentiation process in the whitefly Bemisia tabaci AsiaII-1. Gene. 850, p. 146929. https://doi.org/10.1016/j.gene.2022.146929

Publication dates
Authors	Brar, G. S., Singh, S., Shukla, J. N., Kumar, V. J., Davies, T. G. E., Kaur, G., Pandher, S and Kaur, R.
Abstract	The silverleaf whitefly Bemisia tabaci is one of the most destructive of crop pests globally. In Northern India cotton is predominately infested by the Asia II-1 species of B. tabaci. Though B. tabaci exhibits unique haplodiploidy in its reproductive behavior, to date very little is known regarding its sex determination mechanism. Here, an in-depth characterization of the AsiaII-1 doublesex (Btdsx) gene, which has been implicated in sex determination in B. tabaci, indicates the inclusion of six exons and five introns. The pre-mRNA is shown to sex-specifically splice, producing four male isoforms and one female isoform. These BtDsx proteins share common DNA binding (OD1) domains whereas they differ at their C-termini. RT-qPCR analysis revealed a significantly higher expression of Btdsx in female adults compared to that in male adults and earlier developmental stages. Functional characterization of Btdsx through RNA interference (RNAi) resulted in a significant reduction in its expression in both sexes. Btdsx knockdown concomitantly resulted in up-regulation of the expression of vitellogenin (vg) and vitellogenin receptor (vgr) genes in males and their down-regulation in females. Btdsx knockdown followed by mating resulted in reduced fecundity and percent egg hatching; however, no impact was observed on the female: male ratios in the progeny obtained from knockdown parents.
Keywords	Bemisia tabaci; Doublesex; Sex specific splicing; DsRNA feeding
Year of Publication	2022
Journal	Gene
Journal citation	850, p. 146929
Digital Object Identifier (DOI)	https://doi.org/10.1016/j.gene.2022.146929
PubMed Central ID	36183921
Web address (URL)	https://www.sciencedirect.com/science/article/pii/S0378111922007491
Open access	Published as non-open access
Funder	Rothamsted Research
Funder project or code	Rothamsted international fellowship
Supplemental file	1-s2.0-S0378111922007491-mmc1.pdf
Output status	Published
Online	30 Sep 2022
Publication process dates
Accepted	26 Sep 2022
Publisher	Elsevier Science Bv
ISSN	0378-1119

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Identification of ion channel genes in the Acyrthosiphon pisum genome

A - Papers appearing in refereed journals

Dale, R. P., Jones, A. K., Tamborindeguy, C., Davies, T. G. E., Amey, J. S., Williamson, S., Wolstenholme, A., Field, L. M., Williamson, M. S., Walsh, T. K. and Sattelle, D. B. 2010. Identification of ion channel genes in the Acyrthosiphon pisum genome. Insect Molecular Biology. 19(Suppl. 2), pp. 141-153. https://doi.org/10.1111/j.1365-2583.2009.00975.x

Interactions of pyrethroids with the voltage-gated sodium channel

G - Articles in popular magazines and other technical publications

Davies, T. G. E. and Williamson, M. S. 2009. Interactions of pyrethroids with the voltage-gated sodium channel. Bayer Crop Science Journal. 62, pp. 159-178.

Molecular identification and characterisation of the Myzus persicae voltage gated sodium channel gene

C1 - Edited contributions to conferences/learned societies

Amey, J., O'reilly, A., Williamson, M. S., Field, L. M., Wallace, B. A. and Davies, T. G. E. 2009. Molecular identification and characterisation of the Myzus persicae voltage gated sodium channel gene.

Single channel study of deltamethrin interactions with wild-type and mutated rat Nav1.2 sodium channels expressed in Xenopus oocytes

A - Papers appearing in refereed journals

Peng, F., Mellor, I. R., Williamson, M. S., Davies, T. G. E., Field, L. M. and Usherwood, P. N. R. 2009. Single channel study of deltamethrin interactions with wild-type and mutated rat Nav1.2 sodium channels expressed in Xenopus oocytes. Neurotoxicology. 30, pp. 358-367. https://doi.org/10.1016/j.neuro.2009.02.016

Knockdown resistance to DDT and pyrethroids: from target-site mutations to molecular modelling

A - Papers appearing in refereed journals

Davies, T. G. E., O'Reilly, A. O., Field, L. M., Wallace, B. A. and Williamson, M. S. 2008. Knockdown resistance to DDT and pyrethroids: from target-site mutations to molecular modelling. Pest Management Science. 64 (11), pp. 1126-1130. https://doi.org/10.1002/ps.1617

Mutations in DIIS5 and the DIIS4-S5 linker of Drosophila melanogaster sodium channel define binding domains for pyrethroids and DDT

A - Papers appearing in refereed journals

Usherwood, P. N. R., Davies, T. G. E., Mellor, I. R., O'Reilly, A. O., Peng, F., Vais, H., Khambay, B. P. S., Field, L. M. and Williamson, M. S. 2007. Mutations in DIIS5 and the DIIS4-S5 linker of Drosophila melanogaster sodium channel define binding domains for pyrethroids and DDT. FEBS Letters. 581 (28), pp. 5485-5492. https://doi.org/10.1016/j.febslet.2007.10.057

DDT, pyrethrins, pyrethroids and insect sodium channels

A - Papers appearing in refereed journals

Davies, T. G. E., Field, L. M., Usherwood, P. N. R. and Williamson, M. S. 2007. DDT, pyrethrins, pyrethroids and insect sodium channels. IUBMB Life. 59 (3), pp. 151-162. https://doi.org/10.1080/15216540701352042

A comparative study of voltage-gated sodium channels in the Insecta: implications for pyrethroid resistance in Anopheline and other Neopteran species

A - Papers appearing in refereed journals

Davies, T. G. E., Field, L. M., Usherwood, P. N. R. and Williamson, M. S. 2007. A comparative study of voltage-gated sodium channels in the Insecta: implications for pyrethroid resistance in Anopheline and other Neopteran species. Insect Molecular Biology. 16 (3), pp. 361-375. https://doi.org/10.1111/j.1365-2583.2007.00733.x

Modelling insecticide-binding sites in the voltage-gated sodium channel

A - Papers appearing in refereed journals

O'Reilly, A. O., Khambay, B. P. S., Williamson, M. S., Field, L. M., Wallace, B. A. and Davies, T. G. E. 2006. Modelling insecticide-binding sites in the voltage-gated sodium channel. Biochemical Journal. 396, pp. 255-263. https://doi.org/10.1042/BJ20051925

Sensitivity of the Drosophila para sodium channel to DDT is not lowered by the super-kdr mutation M918T on the IIS4-S5 linker that profoundly reduces sensitivity to permethrin and deltamethrin

A - Papers appearing in refereed journals

Usherwood, P. N. R., Vais, H., Khambay, B. P. S., Davies, T. G. E. and Williamson, M. S. 2005. Sensitivity of the Drosophila para sodium channel to DDT is not lowered by the super-kdr mutation M918T on the IIS4-S5 linker that profoundly reduces sensitivity to permethrin and deltamethrin. FEBS Letters. 579 (28), pp. 6317-6325. https://doi.org/10.1016/j.febslet.2005.09.096

Development of an insect cell line expression system for para sodium channels

C2 - Non-edited contributions to conferences

Davies, T. G. E. and Williamson, M. S. 2003. Development of an insect cell line expression system for para sodium channels. Abstracts Neurotox 2003: Neurotoxicological Targets from Functional Genomics and Proteomics, Nottingham, 1-4 September 2003 .

Restricted spatial expression of a high-affinity phosphate transporter in potato roots

A - Papers appearing in refereed journals

Gordon-Weeks, R., Tong, Y., Davies, T. G. E. and Leggewie, G. 2003. Restricted spatial expression of a high-affinity phosphate transporter in potato roots. Journal Of Cell Science. 116 (15), pp. 3135-3144. https://doi.org/10.1242/jcs.00615

Expression analysis of putative high-affinity phosphate transporters in Chinese winter wheats

A - Papers appearing in refereed journals

Davies, T. G. E., Ying, J., Xu, Q., Li, Z. S., Li, J. and Gordon-Weeks, R. 2002. Expression analysis of putative high-affinity phosphate transporters in Chinese winter wheats. Plant, Cell and Environment. 25 (10), pp. 1325-1339. https://doi.org/10.1046/j.1365-3040.2002.00913.x

Triticum aestivum partial mRNA for putative inorganic phosphate transporter (pt8 gene)

K - Protein or DNA sequences submitted to database

Davies, T. G. E. 2001. Triticum aestivum partial mRNA for putative inorganic phosphate transporter (pt8 gene).

Triticum aestivum partial mRNA for inorganic phosphate transporter (pt7 gene)

K - Protein or DNA sequences submitted to database

Davies, T. G. E. 2001. Triticum aestivum partial mRNA for inorganic phosphate transporter (pt7 gene).

Triticum aestivum partial mRNA for inorganic phosphate transporter (pt6 gene)

K - Protein or DNA sequences submitted to database

Davies, T. G. E. 2001. Triticum aestivum partial mRNA for inorganic phosphate transporter (pt6 gene).

Unlocking soil phosphorus with the help of P efficient wheat varieties from China

G - Articles in popular magazines and other technical publications

Gordon-Weeks, R., Davies, T. G. E. and Barraclough, P. B. 2001. Unlocking soil phosphorus with the help of P efficient wheat varieties from China. BBSRC Business. January 2001, pp. 20-21.

Localisation of high affinity phosphate transporters

C2 - Non-edited contributions to conferences

Gordon-Weeks, R., Davies, T. G. E., Xu, Q., Ying, J. and Li, Z-S. 2001. Localisation of high affinity phosphate transporters. Proceedings 12th International Workshop on Plant Membrane Biology, Madison, 11-16 August 2001 . pp. 216

Triticum aestivum partial mRNA for putative inorganic phosphate transporter, (pt5 gene), clone 2

K - Protein or DNA sequences submitted to database

Davies, T. G. E. 2001. Triticum aestivum partial mRNA for putative inorganic phosphate transporter, (pt5 gene), clone 2.

The Arabidopsis thaliana ABC protein superfamily: a complete inventory

C2 - Non-edited contributions to conferences

Sanchez-Fernandez, R., Davies, T. G. E., Coleman, J. O. D. and Rea, P. A. 2001. The Arabidopsis thaliana ABC protein superfamily: a complete inventory. Abstracts 12th International Workshop on Plant Membrane Biology, Madison, 11-16 August 2001 . pp. 77

The Arabidopsis thaliana ABC protein superfamily: a complete inventory

A - Papers appearing in refereed journals

Sanchez-Fernandez, R., Davies, T. G. E., Coleman, J. O. D. and Rea, P. A. 2001. The Arabidopsis thaliana ABC protein superfamily: a complete inventory. Journal of Biological Chemistry. 276 (32), pp. 30231-30244. https://doi.org/10.1074/jbc.M103104200

Complete inventory of Arabidopsis ABC proteins

C2 - Non-edited contributions to conferences

Sanchez-Fernandez, R., Davies, T. G. E., Coleman, J. O. D. and Rea, P. A. 2001. Complete inventory of Arabidopsis ABC proteins. 3rd FEBS Advanced Lecture Course on ATP-Binding Cassette (ABC) Proteins: from Multidrug Resistance to Genetic Disease, Gosau, Austria, 3-10 March 2001 .

Do plants have more genes than humans? Yes when it comes to ABC proteins

A - Papers appearing in refereed journals

Sanchez-Fernandez, R., Rea, P. A., Davies, T. G. E. and Coleman, J. O. D. 2001. Do plants have more genes than humans? Yes when it comes to ABC proteins. Trends in Plant Science. 6 (8), pp. 347-348. https://doi.org/10.1016/S1360-1385(01)02038-6

ArabidopsisABC.Net (Web site at http://www.arabidopsisabc.net)

G - Articles in popular magazines and other technical publications

Sanchez-Fernandez, R., Rea, P. A., Davies, T. G. E. and Coleman, J. O. D. 2001. ArabidopsisABC.Net (Web site at http://www.arabidopsisabc.net).

Do plants have more genes than humans? Yes when it comes to ABC proteins (Online preview of paper to be published in Trends in Plant Science )

G - Articles in popular magazines and other technical publications

Sanchez-Fernandez, R., Rea, P. A., Davies, T. G. E. and Coleman, J. O. D. 2001. Do plants have more genes than humans? Yes when it comes to ABC proteins (Online preview of paper to be published in Trends in Plant Science ). HMS Beagle. 106.

Physcomitrella patens partial vp1 gene for vacuolar pyrophosphatase, exons 1-3

K - Protein or DNA sequences submitted to database

Davies, T. G. E. 2001. Physcomitrella patens partial vp1 gene for vacuolar pyrophosphatase, exons 1-3.

Physcomitrella patens partial mRNA for vacuolar pyrophosphatase (vp1 gene)

K - Protein or DNA sequences submitted to database

Davies, T. G. E. 2001. Physcomitrella patens partial mRNA for vacuolar pyrophosphatase (vp1 gene).

The Arabidopsis thaliana ATP-binding cassette proteins: an emerging superfamily

A - Papers appearing in refereed journals

Davies, T. G. E. and Coleman, J. O. D. 2000. The Arabidopsis thaliana ATP-binding cassette proteins: an emerging superfamily. Plant, Cell and Environment. 23 (5), pp. 431-443. https://doi.org/10.1046/j.1365-3040.2000.00577.x

ATP-binding cassette proteins of Arabidopsis thaliana

C2 - Non-edited contributions to conferences

Davies, T. G. E. and Coleman, J. O. D. 2000. ATP-binding cassette proteins of Arabidopsis thaliana. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Hatfield, 6-9 September 2000 .

Isolation of an Arabidopsis AtPT2 transposon insertion mutant from the John Innes SLAT collection

C2 - Non-edited contributions to conferences

Davies, T. G. E. and Gordon-Weeks, R. 2000. Isolation of an Arabidopsis AtPT2 transposon insertion mutant from the John Innes SLAT collection. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Hatfield, 6-9 September 2000 .

Characterisation of high-affinity phosphate transporter homologues from phosphate-efficient Chinese wheat varieties

C2 - Non-edited contributions to conferences

Davies, T. G. E., Xu, Q., Li, Z. and Gordon-Weeks, R. 2000. Characterisation of high-affinity phosphate transporter homologues from phosphate-efficient Chinese wheat varieties. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Hatfield, 6-9 September 2000 .

ATP-binding cassette proteins of Arabidopsis thaliana

C2 - Non-edited contributions to conferences

Davies, T. G. E. and Coleman, J. O. D. 2000. ATP-binding cassette proteins of Arabidopsis thaliana. Abstracts COST Action 837 Workshop on Phytoremediation 2000 - State of the Art in Europe - an Intercontinental Comparison, Athens, 6-8 April 2000 . pp. 148

Biochemical characterisation of plant plasma membrane phosphate transporters

C2 - Non-edited contributions to conferences

Gordon-Weeks, R., Leggewie, G. and Davies, T. G. E. 1999. Biochemical characterisation of plant plasma membrane phosphate transporters. InAbstracts Society for Experimental Biology Plant Transport Group Meeting, Southampton, 8-10 September 1999 .

Structural aspects of the effectiveness of bisphosphonates as competitive inhibitors of the plant vacuolar proton-pumping pyrophosphatase

A - Papers appearing in refereed journals

Gordon-Weeks, R., Parmar, S., Davies, T. G. E. and Leigh, R. A. 1999. Structural aspects of the effectiveness of bisphosphonates as competitive inhibitors of the plant vacuolar proton-pumping pyrophosphatase. Biochemical Journal. 337, pp. 373-377.

Are P-glycoproteins in plants chloride channel regulators?

C2 - Non-edited contributions to conferences

Davies, T. G. E. 1998. Are P-glycoproteins in plants chloride channel regulators? Abstracts 9th Colloque Canaux Ioniques, La Londe les Maures, 1998 . pp. P42

Molecular approaches towards elucidation of the function of the vacuolar H+-pumping inorganic pyrophosphatase in plants

C2 - Non-edited contributions to conferences

Davies, T. G. E., Gordon-Weeks, R., Zrenner, R., Stitt, M., Knight, C. D., Wang, T. L. and Leigh, R. A. 1998. Molecular approaches towards elucidation of the function of the vacuolar H+-pumping inorganic pyrophosphatase in plants. Abstracts 11th International Workshop on Plant Membrane Biology, Cambridge, 9-14 August 1998 .

HvMDR 2 - a novel P-glycoprotein homologue from barley

C2 - Non-edited contributions to conferences

Theodoulou, F. L., Davies, T. G. E., Hallahan, D. L. and Forde, B. G. 1998. HvMDR 2 - a novel P-glycoprotein homologue from barley. Abstracts 11th International Workshop on Plant Membrane Biology, Cambridge, 9-14 August 1998 . pp. 78

Pyrophosphate analogues differ in their effectiveness as competitive inhibitors of the plant vacuolar proton-pumping pyrophosphatase.

C2 - Non-edited contributions to conferences

Gordon-Weeks, R., Davies, T. G. E., Parmar, S. and Leigh, R. A. 1998. Pyrophosphate analogues differ in their effectiveness as competitive inhibitors of the plant vacuolar proton-pumping pyrophosphatase. Abstracts 11th International Workshop on Plant Membrane Biology, Cambridge, 9-14 August 1998 . pp. 56

Structure and function of the plant vacuolar H+-pumping inorganic pyrophosphatase

C2 - Non-edited contributions to conferences

Gordon-Weeks, R., Davies, T. G. E., Parmar, S. and Leigh, R. A. 1997. Structure and function of the plant vacuolar H+-pumping inorganic pyrophosphatase. Ecophysiology of Plants: Molecules, Membranes and Mechanisms. Abstracts 2nd International Symposium of the Sonderforschungsbereich 199, Edernburg, Germany, 30 October-2 November 1997 . pp. 30-31

Characterization of a novel P-glycoprotein homologue from barley - an ABC-type transporter with no assigned function in plants

C2 - Non-edited contributions to conferences

Davies, T. G. E. and Forde, B. G. 1997. Characterization of a novel P-glycoprotein homologue from barley - an ABC-type transporter with no assigned function in plants. Abstracts SEB Plant Transport Group Workshop, Glasgow, 8-10 September 1997 . pp. T10

Pyrophosphate analogues differ in their effectiveness as competitive inhibitors of the plant vacuolar proton-pumping pyrophosphatase: a structural explanation

C2 - Non-edited contributions to conferences

Davies, T. G. E., Parmar, S., Gordon-Weeks, R. and Leigh, R. A. 1997. Pyrophosphate analogues differ in their effectiveness as competitive inhibitors of the plant vacuolar proton-pumping pyrophosphatase: a structural explanation. Abstracts SEB Plant Transport Group Workshop, Glasgow, 8-10 September 1997 . pp. T7

Cloning and characterisation of a novel P-glycoprotein homologue from barley

A - Papers appearing in refereed journals

Davies, T. G. E., Theodoulou, F. L., Hallahan, D. L. and Forde, B. G. 1997. Cloning and characterisation of a novel P-glycoprotein homologue from barley. Gene. 199, pp. 195-202.

Detoxification of xenobiotics by plants: chemical modification and vacuolar compartmentation

A - Papers appearing in refereed journals

Coleman, J. O. D., Blake-Kalff, M. M. A. and Davies, T. G. E. 1997. Detoxification of xenobiotics by plants: chemical modification and vacuolar compartmentation. Trends in Plant Science. 2 (4), pp. 144-151. https://doi.org/10.1046/j.1365-3040.1997.d01-93.x

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