Sensitivity of the Drosophila para sodium channel to DDT is not lowered by the super-kdr mutation M918T on the IIS4-S5 linker that profoundly reduces sensitivity to permethrin and deltamethrin

A - Papers appearing in refereed journals

Usherwood, P. N. R., Vais, H., Khambay, B. P. S., Davies, T. G. E. and Williamson, M. S. 2005. Sensitivity of the Drosophila para sodium channel to DDT is not lowered by the super-kdr mutation M918T on the IIS4-S5 linker that profoundly reduces sensitivity to permethrin and deltamethrin. FEBS Letters. 579 (28), pp. 6317-6325.

AuthorsUsherwood, P. N. R., Vais, H., Khambay, B. P. S., Davies, T. G. E. and Williamson, M. S.
Abstract

DDT inhibits Na channel inactivation and deactivation, promotes Na channel activation and reduces the resting potential of Xenopus oocytes expressing the Drosophila para Na channel. These changes are only marginally influenced by the single mutation M918T (super-kdr) but are reduced similar to 10-fold by either the single mutation L1014F (kdr) or the double mutation L1014F + M918T, both of which confer resistance to the pyre-throids permethrin and deltamethrin. We conclude that DDT binds either to or in the region of L1014 on IIS6 but only weakly to M918 on the IIS4-S5 linker, which is part of a high-affinity binding site for permethrin and deltamethrin. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KeywordsBiochemistry & Molecular Biology; Biophysics; Cell Biology
Year of Publication2005
JournalFEBS Letters
Journal citation579 (28), pp. 6317-6325
Digital Object Identifier (DOI)doi:10.1016/j.febslet.2005.09.096
PubMed ID16263118
Open accessPublished as non-open access
Funder project or code514
The molecular basis of target site and metabolic insecticide resistance
ISSN00145793
PublisherWiley

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