Identification of pyrethroid resistance associated mutations in the para sodium channel of the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae)

A - Papers appearing in refereed journals

Tsagkarakou, A., Van Leeuwen, T., Khajehali, J., Ilias, A., Grispou, M., Williamson, M. S., Tirry, L. and Vontas, J. 2009. Identification of pyrethroid resistance associated mutations in the para sodium channel of the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae). Insect Molecular Biology. 18 (5), pp. 583-593.

AuthorsTsagkarakou, A., Van Leeuwen, T., Khajehali, J., Ilias, A., Grispou, M., Williamson, M. S., Tirry, L. and Vontas, J.
Abstract

We investigated pyrethroid resistance mechanisms in Tetranychus urticae strains from Greece. Combined bioassay, biochemical and synergistic data indicated that although P450 mono-oxygenase activities were associated with the trait, target site insensitivity was the major resistance component. A 3.3 kb cDNA fragment of the T. urticae para sodium channel gene encompassing segment 4 of domain II to segment 6 of domain IV was obtained by a degenerate PCR strategy. The T. urticae sequence showed highest identity (56%) to the scabies mite, Sarcoptes scabiei, and was phylogenetically classified within the divergent group of Arachnida. Comparison of resistant and susceptible strains identified the point mutation F1538I in segment 6 of domain III, which is known to confer strong resistance to pyrethroids, along with a second mutation (A1215D) in the intracellular linker connecting domains II and III with an unknown role. Three transcripts were identified corresponding to the k and l alternative exons. The mode of inheritance of resistance was confirmed as incompletely recessive, which is consistent with a target site mechanism for pyrethroids.

KeywordsBiochemistry & Molecular Biology; Entomology
Year of Publication2009
JournalInsect Molecular Biology
Journal citation18 (5), pp. 583-593
Digital Object Identifier (DOI)doi:10.1111/j.1365-2583.2009.00900.x
PubMed ID19754737
Open accessPublished as non-open access
FunderResearch Foundation Flanders (Belgium)
Funder project or codePDM
ISSN09621075
PublisherWiley

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