A sodium channel point mutation is associated with resistance to DDT and pyrethroid insecticides in the peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae)

A - Papers appearing in refereed journals

Martinez-Torres, D., Foster, S. P., Field, L. M., Devonshire, A. L. and Williamson, M. S. 1999. A sodium channel point mutation is associated with resistance to DDT and pyrethroid insecticides in the peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Insect Molecular Biology. 8 (3), pp. 339-346.

AuthorsMartinez-Torres, D., Foster, S. P., Field, L. M., Devonshire, A. L. and Williamson, M. S.
Abstract

The voltage-gated sodium channel is the primary target site of DDT and pyrethroid insecticides, and point mutations in the domain II region of the channel protein have been implicated in the knockdown resistant (kdr) phenotype of several insect species. Here, we report that one of these mutations, a leucine-to-phenylalanine replacement in transmembrane segment IIS6, is also found in certain insecticide-resistant clones of the peach-potato aphid, Myzus persicae, The mutation was present in four clones with amplified E4 esterase genes, but was absent from both susceptible clones and those with amplified FE4 genes. The inferred presence of kdr-type resistance in the four E4 clones was subsequently confirmed by bioassays that showed this to be the primary mechanism of resistance to deltamethrin and DDT, although the esterase-based mechanism also contributes to the overall level of deltamethrin resistance. The kdr mutation on its own conferred 35-fold resistance to deltamethrin and this was enhanced up to 540-fold when it was present in a high (E4) esterase background. The esterase (FE4) mechanism was far less effective without the kdr mutation, conferring just 3-4-fold resistance to deltamethrin. These findings, and the linkage disequilibrium of the kdr mutation within clones overproducing the E4 esterase, have important implications for the evolution of resistance in this insect and for the use of pyrethroid sprays in the management of M. persicae populations in the field.

KeywordsBiochemistry & Molecular Biology; Entomology
Year of Publication1999
JournalInsect Molecular Biology
Journal citation8 (3), pp. 339-346
Digital Object Identifier (DOI)doi:10.1046/j.1365-2583.1999.83121.x
PubMed ID10469251
Open accessPublished as non-open access
Funder project or code438
ISSN09621075
PublisherWiley

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