Crystal structures and binding dynamics of odorant-binding protein 3 from two aphid species Megoura viciae and Nasonovia ribisnigri

A - Papers appearing in refereed journals

Northey, T., Venthur, H., De Biasio, F., Chauviac, F-X., Cole, A., Ribeiro, K. A. L. Junior, Grossi, G., Falabella, P., Field, L. M., Keep, N. H. and Zhou, J-J. 2016. Crystal structures and binding dynamics of odorant-binding protein 3 from two aphid species Megoura viciae and Nasonovia ribisnigri. Scientific Reports. 6, p. 24739. https://doi.org/10.1038/srep24739

AuthorsNorthey, T., Venthur, H., De Biasio, F., Chauviac, F-X., Cole, A., Ribeiro, K. A. L. Junior, Grossi, G., Falabella, P., Field, L. M., Keep, N. H. and Zhou, J-J.
Abstract

Aphids use chemical cues to locate hosts and find mates. The vetch aphid Megoura viciae feeds exclusively on the Fabaceae, whereas the currant-lettuce aphid Nasonovia ribisnigri alternates hosts between the Grossulariaceae and Asteraceae. Both species use alarm pheromones to warn of dangers. For N. ribisnigri this pheromone is a single component (E)-β-farnesene but M. viciae uses a mixture of (E)-β-farnesene, (−)-α-pinene, β-pinene, and limonene. Odorant-binding proteins (OBP) are believed to capture and transport such semiochemicals to their receptors. Here, we report the first aphid OBP crystal structures and examine their molecular interactions with the alarm pheromone components. Our study reveals some unique structural features: 1) the lack of an internal ligand binding site; 2) a striking groove in the surface of the proteins as a putative binding site; 3) the N-terminus rather than the C-terminus occupies the site closing off the conventional OBP pocket. The results from fluorescent binding assays, molecular docking and dynamics demonstrate that OBP3 from M. viciae can bind to all four alarm pheromone components and the differential ligand binding between these very similar OBP3s from the two aphid species is determined mainly by the direct π-π interactions between ligands and the aromatic residues of OBP3s in the binding pocket.

Year of Publication2016
JournalScientific Reports
Journal citation6, p. 24739
Digital Object Identifier (DOI)https://doi.org/10.1038/srep24739
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Royal Society
Funder project or codeDelivering Sustainable Systems (SS) [ISPG]
UK-China Collaboration for production of biologically active homoisoprenoids and their eventual use for pest control
New pest resistance in rice, by breeding and genetic modification (GM) for constitutive and inducible levels of defence homoterpenes
JP100849
Innovative approaches to pest management
Publisher's version
Copyright license
CC BY
Output statusPublished
Publication dates
Online22 Apr 2016
Publication process dates
Accepted05 Apr 2016
PublisherSpringer Nature
Nature Publishing Group
ISSN2045-2322

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