Expression of chemosensory proteins in the tsetse fly Glossina morsitans morsitans is related to female host-seeking behaviour

A - Papers appearing in refereed journals

Liu, R., He, X., Lehane, S., Lehane, M., Hertz-Fowler, C., Berriman, M., Field, L. M. and Zhou, J-J. 2012. Expression of chemosensory proteins in the tsetse fly Glossina morsitans morsitans is related to female host-seeking behaviour. Insect Molecular Biology. 21 (1), pp. 41-48.

AuthorsLiu, R., He, X., Lehane, S., Lehane, M., Hertz-Fowler, C., Berriman, M., Field, L. M. and Zhou, J-J.
Abstract

Chemosensory proteins (CSPs) are a class of soluble proteins present in high concentrations in the sensilla of insect antennae. It has been proposed that they play an important role in insect olfaction by mediating interactions between odorants and odorant receptors. Here we report, for the first time, the presence of five CSP genes in the tsetse fly Glossina morsitans morsitans, a major vector transmitting nagana in livestock. Real-time quantitative reverse transcription PCR showed that three of the CSPs are expressed in antennae. One of them, GmmCSP2, is transcribed at a very high level and could be involved in olfaction. We also determined expression in the antennae of both males and females at different life stages and with different blood feeding regimes. The transcription of GmmCSP2 was lower in male antennae than in females, with a sharp increase in 10-week-old flies, 48 h after a bloodmeal. Thus there is a clear relationship between CSP gene transcription and host searching behaviour. Genome annotation and phylogenetic analyses comparing G. morsitans morsitans CSPs with those of other Diptera showed rapid evolution after speciation of mosquitoes.

KeywordsBiochemistry ; Entomology; Molecular biology
Year of Publication2012
JournalInsect Molecular Biology
Journal citation21 (1), pp. 41-48
Digital Object Identifier (DOI)doi:10.1111/j.1365-2583.2011.01114.x
PubMed ID22074189
Open accessPublished as green open access
FunderEuropean Commission - EC
Wellcome Trust
Biotechnology and Biological Sciences Research Council
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
Project: 4344
Publisher's version
PublisherWiley
Grant IDWT085775/Z/08/Z
ISSN0962-1075

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