Molecular identification and characterization of the Orco orthologue of Spodoptera litura

A - Papers appearing in refereed journals

Wu, Z-N., Chen, X., Du, Y-J., Zhou, J-J. and Zhuge, Q-C. 2013. Molecular identification and characterization of the Orco orthologue of Spodoptera litura. Insect Science. 20 (2), pp. 175-182.

AuthorsWu, Z-N., Chen, X., Du, Y-J., Zhou, J-J. and Zhuge, Q-C.
Abstract

A highly conserved and broadly expressed receptor protein Orco (olfactory coreceptor) is crucial for insect olfaction, and an orthologue of Orco has been identified in several insect species. Here we report the identification and characterization of Orco from Spodoptera litura. The protein displays high primary amino acid sequence conservation with other previously identified Orco orthologues. Bioinformatic analysis revealed that it has common features with other members of the Orco subfamily: seven-transmembrane domains with intracellular N-terminus and extracellular C-terminus. The transcript was detected in abundance in the chemosensory organs of the antennae of both male and female adults by real-time polymerase chain reaction analysis, and was localized at the bases of all categories of olfactory sensilla through in situ hybridization.

KeywordsEntomology
Year of Publication2013
JournalInsect Science
Journal citation20 (2), pp. 175-182
Digital Object Identifier (DOI)doi:10.1111/j.1744-7917.2011.01483.x
PubMed ID23955858
Open accessPublished as non-open access
FunderZhejiang Provincial Natural Science Foundation of China
Foundation of Zhejiang Educational Committee
Funder project or codeDelivering Sustainable Systems (SS) [ISPG]
Innovative approaches to pest management
PublisherWiley
Grant IDD3080388
Y200803904
ISSN1672-9609

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