A - Papers appearing in refereed journals
Khot, A. C., Bingham, G., Field, L. M. and Moores, G. D. 2008. A novel assay reveals the blockade of esterases by piperonyl butoxide. Pest Management Science. 64 (11), pp. 1139-1142. https://doi.org/10.1002/ps.1603
Authors | Khot, A. C., Bingham, G., Field, L. M. and Moores, G. D. |
---|---|
Abstract | BACKGROUND: Conventional in vitro assays sometimes fail to reveal esterase inhibition by piperonyl butoxide (PBO), although synergism studies suggest loss of esterase-mediated sequestration of insecticide does take place. A new in vitro assay has been devised that routinely reveals binding between PBO and these esterases. RESULTS: The new 'esterase interference' assay detects the blockade of resistance-associated esterases in a species, Myzus persicae Sulzer, where this has not previously been seen. The assay also demonstrates directly the protective effect esterases may confer to target sites of insecticides. CONCLUSION: The new assay reveals esterase blockade by PBO and thus has the potential to be used as a high-throughput screening method for other potential synergists. (c) 2008 Society of Chemical Industry |
Keywords | Agronomy; Entomology |
Year of Publication | 2008 |
Journal | Pest Management Science |
Journal citation | 64 (11), pp. 1139-1142 |
Digital Object Identifier (DOI) | https://doi.org/10.1002/ps.1603 |
PubMed ID | 18481337 |
Open access | Published as non-open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Centre for Sustainable Pest and Disease Management (PDM) |
ISSN | 1526498X |
Publisher | Wiley |
Permalink - https://repository.rothamsted.ac.uk/item/8q095/a-novel-assay-reveals-the-blockade-of-esterases-by-piperonyl-butoxide