A novel assay reveals the blockade of esterases by piperonyl butoxide

A - Papers appearing in refereed journals

Khot, A. C., Bingham, G., Field, L. M. and Moores, G. D. 2008. A novel assay reveals the blockade of esterases by piperonyl butoxide. Pest Management Science. 64 (11), pp. 1139-1142.

AuthorsKhot, A. C., Bingham, G., Field, L. M. and Moores, G. D.
Abstract

BACKGROUND: Conventional in vitro assays sometimes fail to reveal esterase inhibition by piperonyl butoxide (PBO), although synergism studies suggest loss of esterase-mediated sequestration of insecticide does take place. A new in vitro assay has been devised that routinely reveals binding between PBO and these esterases. RESULTS: The new 'esterase interference' assay detects the blockade of resistance-associated esterases in a species, Myzus persicae Sulzer, where this has not previously been seen. The assay also demonstrates directly the protective effect esterases may confer to target sites of insecticides. CONCLUSION: The new assay reveals esterase blockade by PBO and thus has the potential to be used as a high-throughput screening method for other potential synergists. (c) 2008 Society of Chemical Industry

KeywordsAgronomy; Entomology
Year of Publication2008
JournalPest Management Science
Journal citation64 (11), pp. 1139-1142
Digital Object Identifier (DOI)doi:10.1002/ps.1603
PubMed ID18481337
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
ISSN1526498X
PublisherWiley

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