Esterase isoenzymes and insecticide resistance in Frankliniella occidentalis populations from the south-east region of Spain

A - Papers appearing in refereed journals

Lopez-Soler, N., Cervera, A., Moores, G. D., Martinez-Pardo, R. and Garcera, M. D. 2008. Esterase isoenzymes and insecticide resistance in Frankliniella occidentalis populations from the south-east region of Spain. Pest Management Science. 64 (12), pp. 1258-1266.

AuthorsLopez-Soler, N., Cervera, A., Moores, G. D., Martinez-Pardo, R. and Garcera, M. D.
Abstract

BACKGROUND: Frankliniella occidentalis (Pergande) is among the most important crop pests in the south-east region of Spain; its increasing resistance to insecticides constitutes a serious problem, and understanding the mechanisms involved is therefore of great interest. To this end, F. occidentalis populations, collected from the field at different locations in south-east Spain, were studied in terms of total esterase activity and esterase isoenzyme pattern. RESULTS: Individual thrips extracts were analysed by native polyacrylamide gel electrophoresis (PAGE) and stained for esterase activity with the model substrate alpha-naphthyl acetate. Significant correlations were found between resistance to the insecticides acrinathrin and methiocarb and the presence of a group of three intensely stained bands, named Triplet A. For each individual thrips extract, total esterase activity towards the substrates alpha-naphthyl acetate and a-naphthyl butyrate was also measured in a microplate reader. Insects possessing Triplet A showed a significantly higher a-naphthyl acetate specific activity and a-naphthyl acetate/alpha-naphthyl butyrate activity ratio. This observation allowed a reliable classification of susceptible or resistant insects either by PAGE analysis or by total esterase activity determination. CONCLUSION: The PAGE and microplate assays described can be used as a monitoring technique for detecting acrinathrin- and methiocarb-resistant individuals among F. occidentalis field populations. (C) 2008 Society of Chemical Industry

KeywordsAgronomy; Entomology
Year of Publication2008
JournalPest Management Science
Journal citation64 (12), pp. 1258-1266
Digital Object Identifier (DOI)doi:10.1002/ps.1627
PubMed ID18688781
Open accessPublished as non-open access
FunderMinisterio de Ciencia y Tecnologia
Ministerio de Educacion y Ciencia, Spain
FEDER
Departamento de Produccion Agraria (Universidad Politecnica de Cartagena, Spain)
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
The molecular basis of target site and metabolic insecticide resistance
ISSN1526498X
PublisherWiley
Grant IDAGL2002-04190-C02-01
AGL2005-07492-C02-02

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