The evolution of multiple-insecticide resistance in UK populations of tomato leafminer, Tuta absoluta

A - Papers appearing in refereed journals

Grant, C., Jacobson, R., Ilias, A., Berger, M., Vasakis, E., Bielza, P., Zimmer, C. T., Williamson, M. S., Ffrench-Constant, R. H., Vontas, J., Roditakis, E. and Bass, C. 2019. The evolution of multiple-insecticide resistance in UK populations of tomato leafminer, Tuta absoluta. Pest Management Science.

AuthorsGrant, C., Jacobson, R., Ilias, A., Berger, M., Vasakis, E., Bielza, P., Zimmer, C. T., Williamson, M. S., Ffrench-Constant, R. H., Vontas, J., Roditakis, E. and Bass, C.
Abstract

The tomato leafminer, Tuta absoluta, is an economically important pest of tomatoes in Europe, Africa, Asia and South America. In the UK this species is controlled using an integrated pestmanagement (IPM) programme which incorporates the insecticides spinosad and chlorantraniliprole. In response to UK grower concerns of loss of efficacy of these compounds at certain sites, insecticide bioassays were performed on five populations collected from four commercial glasshouses and potential mechanisms of resistance investigated. We observed high levels of resistance to spinosad in four of the strains, and in two of these tolerance to chlorantraniliprole. Selection of one of these strains with chlorantraniliprole rapidly resulted in a line exhibiting potent resistance to this compound. Sequencing ofmessenger RNAencoding the nicotinic acetylcholine receptor (nAChR)𝜶6 subunit, target of spinosad, revealed Ta𝜶6 transcripts in the spinosad-resistant strains that lack exon 4 and encode a highly truncated protein, or contain a triplet deletion in the predicted first transmembrane domain resulting in the loss of a highly conserved amino acid. Sequencing of the ryanodine receptor gene, encoding the target of diamide insecticides, of the chlorantraniliprole-selected line revealed an amino acid substitution (G4903V) that has been previously linked to diamide resistance in populations of T. absoluta in the Mediterranean and South America. Taken together our results reveal emerging resistance in UK populations of T. absoluta to two of the most important insecticides used as part of IPM, with significant implications for the control of this species in the UK.

KeywordsSpinosad; Chlorantraniliprole; Resistance; Tomato leafminer
Year of Publication2019
JournalPest Management Science
Digital Object Identifier (DOI)doi:10.1002/ps.5381
Open accessPublished as non-open access
FunderAgriculture and Horticulture Development Board
Biotechnology and Biological Sciences Research Council
Output statusPublished
Publication dates
Print20 Mar 2019
Publication process dates
Accepted20 Feb 2019
PublisherWiley
ISSN1526-498X

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