A single amino acid change makes a rat neuronal sodium channel highly sensitive to pyrethroid insecticides

A - Papers appearing in refereed journals

Vais, H., Atkinson, S., Eldursi, N., Devonshire, A. L., Williamson, M. S. and Usherwood, P. N. R. 2000. A single amino acid change makes a rat neuronal sodium channel highly sensitive to pyrethroid insecticides. FEBS Letters. 470 (2), pp. 135-138.

AuthorsVais, H., Atkinson, S., Eldursi, N., Devonshire, A. L., Williamson, M. S. and Usherwood, P. N. R.
Abstract

Two amino acid substitutions in a housefly sodium channel, L1014F in domain IIS6 and M918T in the IIS4-S5 linker, have been identified in kdr and super-kdr pyrethroid-resistant phenotypes, respectively. Unlike their native insect counterparts, mammalian sodium channels are only weakly sensitive to pyrethroids. Do the sodium channels of mammal and pyrethroid-resistant housefly share similar structural characteristics that account for their low pyrethroid sensitivities? We report here that substitution of isoleucine for methionine at position 874 (equivalent to the super-kdr site 918 in the housefly) in the rat IIa alpha-subunit causes a 100-fold increase in sensitivity. (C) 2000 Federation of European Biochemical Societies.

KeywordsBiochemistry & Molecular Biology; Biophysics; Cell Biology
Year of Publication2000
JournalFEBS Letters
Journal citation470 (2), pp. 135-138
Digital Object Identifier (DOI)doi:10.1016/S0014-5793(00)01305-3
PubMed ID10734222
Open accessPublished as non-open access
Funder project or code438
ISSN00145793
PublisherWiley

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