Temperature and nitrogen supply interact to determine protein distribution gradients in the wheat grain endosperm

A - Papers appearing in refereed journals

Savill, G. P., Michalski, A., Powers, S. J., Wan, Y., Tosi, P., Buchner, P. H. and Hawkesford, M. J. 2018. Temperature and nitrogen supply interact to determine protein distribution gradients in the wheat grain endosperm. Journal of Experimental Botany. 69 (12), pp. 3117-3126.

AuthorsSavill, G. P., Michalski, A., Powers, S. J., Wan, Y., Tosi, P., Buchner, P. H. and Hawkesford, M. J.
Abstract

Gradients exist in the distribution of storage proteins in the wheat (Triticum aestivum) endosperm and determine the milling properties and protein recovery rate of the grain. A novel image analysis technique was developed to quantify both the gradients in protein concentration, and the size distribution of protein bodies within the endosperm of wheat plants grown under two different (20 or 28 °C) post-anthesis temperatures, and supplied with a nutrient solution with either high or low nitrogen content. Under all treatment combinations, protein concentration was greater in the endosperm cells closest to the aleurone layer and decreased towards the centre of the two lobes of the grain, i.e. a negative gradient. This was accompanied by a decrease in size of protein bodies from the outer to the inner endosperm layers in all but one of the treatments. Elevated post-anthesis temperature had the effect of increasing the magnitude of the negative gradients in both protein concentration and protein body size, whilst limiting nitrogen supply decreased the gradients.

Keywordsbreadmaking quality; gluten protein; grain protein distribution; protein body size distribution; protein distribution gradient; Triticum aestivum; wheat endosperm; wheat grain protein
Year of Publication2018
JournalJournal of Experimental Botany
Journal citation69 (12), pp. 3117-3126
Digital Object Identifier (DOI)doi:10.1093/jxb/ery127
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or code[20:20 Wheat] Maximising yield potential of wheat
Designing Future Wheat (DFW) [ISPG]
DFW - Designing Future Wheat - Work package 1 (WP1) - Increased efficiency and sustainability
Publisher's version
Output statusPublished
Publication dates
Online05 Apr 2018
Publication process dates
Accepted21 Mar 2018
PublisherOxford University Press (OUP)
Copyright licenseCC BY
ISSN0022-0957

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