A - Papers appearing in refereed journals
Urban, M., Motteram, J., Jing, H-C., Powers, S. J., Townsend, J. A., Devonshire, J., Pearman, I., Kanyuka, K., Franklin, J. F. and Hammond-Kosack, K. E. 2011. Inactivation of plant infecting fungal and viral pathogens to achieve biological containment in drainage water using UV treatment. Journal of Applied Microbiology. 110 (3), pp. 675-687.
|Authors||Urban, M., Motteram, J., Jing, H-C., Powers, S. J., Townsend, J. A., Devonshire, J., Pearman, I., Kanyuka, K., Franklin, J. F. and Hammond-Kosack, K. E.|
Aim: To explore whether ultraviolet (UV) light treatment within a closed circulating and filtered water drainage system can kill plant pathogenic species. Methods and Results: Ultraviolet experiments at 254 nm were conducted to determine the inactivation coefficients for seven plant pathogenic species. At 200 mJ cm-2, the individual species log reductions obtained for six Ascomycete fungi and a cereal virus were as follows: Leptosphaeria maculans (9 center dot 9-log), Leptosphaeria biglobosa (7 center dot 1-log), Barley stripe mosaic virus (BSMV) (4 center dot 1-log), Mycosphaerella graminicola (2 center dot 9-log), Fusarium culmorum (1 center dot 2-log), Fusarium graminearum (0 center dot 6-log) and Magnaporthe oryzae (0 center dot 3-log). Dilution experiments showed that BSMV was rendered noninfectious when diluted to > 1/512. Follow-up large-scale experiments using up to 400 l of microbiologically contaminated waste water revealed that the filtration of drainage water followed by UV treatment could successfully be used to inactivate several plant pathogens. Conclusions: By combining sedimentation, filtration and UV irradiation within a closed system, plant pathogens can be successfully removed from collected drainage water. Significance and Impact of the Study: Ultraviolet irradiation is a relatively low cost, energy efficient and labour nonintensive method to decontaminate water arising from a suite of higher biological containment level laboratories and plant growth rooms where genetically modified and/or quarantine fungal and viral plant pathogenic organisms are being used for research purposes.
|Keywords||Biotechnology & Applied Microbiology; Microbiology|
|Year of Publication||2011|
|Journal||Journal of Applied Microbiology|
|Journal citation||110 (3), pp. 675-687|
|Digital Object Identifier (DOI)||doi:10.1111/j.1365-2672.2010.04917.x|
|Open access||Published as green open access|
|Funder||Biotechnology and Biological Sciences Research Council|
|Funder project or code||Centre for Sustainable Pest and Disease Management (PDM)|
|Centre for Mathematical and Computational Biology (MCB)|
|Pathogenicity of non-biotrophic fungi infecting cereals|
|Application of statistical methods to predictive biology|
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