GCR1 and GPA1 coupling regulates nitrate, cell wall, immunity and light responses in Arabidopsis

A - Papers appearing in refereed journals

Chakraborty, N., Kanyuka, K., Kumar Jaiswal, D., Kumar, A., Arora, V., Malik, A., Gupta, N., Hooley, R. and Raghuram, N. 2019. GCR1 and GPA1 coupling regulates nitrate, cell wall, immunity and light responses in Arabidopsis. Scientific Reports. 9, p. 5838.

AuthorsChakraborty, N., Kanyuka, K., Kumar Jaiswal, D., Kumar, A., Arora, V., Malik, A., Gupta, N., Hooley, R. and Raghuram, N.
Abstract

G-protein signaling components have been attributed many biological roles in plants but the extent of involvement of G-protein coupled receptor 1 (GCR1) with the Gα (GPA1) remained unknown. To address this, we have performed transcriptomic analyses on Arabidopsis gpa1-5gcr1-5 double mutant and identified 656 differentially expressed genes (DEGs). MapMan and Gene Ontology analyses revealed global transcriptional changes associated with external stimulus, cell wall organization/biogenesis and secondary metabolite process among others. Comparative transcriptomic analyses using the single and double mutants of gcr1-5 and gpa1-5 identified 194, 139 and 391 exclusive DEGs respectively, whereas 64 DEGs were common to all three mutants. Further, pair wise comparison of DEGs of double mutant with single mutants of gcr1-5 or gpa1-5 showed about one-third and over half common DEGs, respectively. Further analysis of the DEGs exclusive to the double mutant using protein-protein interaction networks revealed molecular complexes associated with nitrate and light signaling and plant-pathogen interactions among others. Physiological and molecular validation of nitrate-response revealed the sensitivity of germination to low N in the double mutant, and differential expression of nitrate transporter and nitrate reductase in all three mutants. Taken together, GCR1 and GPA1 work in partnership as well as independently to regulate different pathways.

KeywordsG-protein signaling; Nitrate signaling; Cell wall; Immunity; Light responses
Year of Publication2019
JournalScientific Reports
Journal citation9, p. 5838
Digital Object Identifier (DOI)doi:10.1038/s41598-019-42084-2
Web address (URL)https://www.nature.com/articles/s41598-019-42084-2
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience
Publisher's version
Accepted author manuscript
Output statusPublished
Publication dates
Print09 Apr 2019
Publication process dates
Accepted25 Mar 2019
Copyright licenseCC BY
PublisherNature Publishing Group
ISSN2045-2322

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