A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene Silencing (VIGS)

B - Book chapters etc edited externally

Desjardins, S., Kanyuka, K. and Higgins, J. D. 2020. A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene Silencing (VIGS). in: Pradillo, M. and Heckmann, S. (ed.) Plant Meiosis. Methods in Molecular Biology, vol 2061. New York, NY Humana Press Inc. pp. 319-330

AuthorsDesjardins, S., Kanyuka, K. and Higgins, J. D.
EditorsPradillo, M. and Heckmann, S.
Abstract

Virus-induced gene silencing (VIGS) is a rapid and cost-effective reverse genetic technology that can be used to assess gene function in wheat. This chapter contains a detailed description of how to target wheat meiotic genes by VIGS. The timing of this technique is critical and has been optimized to silence meiotic genes at peak expression, evidenced by silencing of Triticum aestivum disrupted meiotic cDNA1 (TaDMC1). We also describe cytological techniques that have been adapted for the preparation and analysis of meiocytes in wheat, including fluorescent in situ hybridization (FISH) with directly labeled, synthetic oligonucleotide probes, and immunolocalization on spread material.

KeywordsVIGS; Meiosis; Cytology; Wheat; FISH; Chromosomes; Immunolocalization
Page range319-330
Year of Publication2020
Book titlePlant Meiosis. Methods in Molecular Biology, vol 2061.
PublisherHumana Press Inc
Place of publicationNew York, NY
ISBN978-1-4939-9817-3
Digital Object Identifier (DOI)doi:10.1007/978-1-4939-9818-0_22
PubMed ID31583669
Web address (URL)http://link.springer.com/10.1007/978-1-4939-9818-0_22
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience
Open accessPublished as non-open access
Publisher's version
Output statusPublished
Publication dates
Print06 Oct 2019
Online04 Oct 2019

Permalink - https://repository.rothamsted.ac.uk/item/96x11/a-cytological-analysis-of-wheat-meiosis-targeted-by-virus-induced-gene-silencing-vigs

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