Virus-mediated protein overexpression (VOX) in monocots to identify and functionally characterize fungal effectors

B - Book chapters etc edited externally

Kanyuka, K. 2022. Virus-mediated protein overexpression (VOX) in monocots to identify and functionally characterize fungal effectors. in: Kufer, T. and Kaparakis-Liaskos, M. (ed.) Effector-triggered immunity Humana Press Inc. pp. 2523 p 93–112

AuthorsKanyuka, K.
EditorsKufer, T. and Kaparakis-Liaskos, M.
Abstract

One of the important armories that plant pathogens utilize to successfully colonize the plants are small secreted effector proteins, which could perform a variety of functions from suppression of plant innate immunity to manipulation of plant physiology in favor of the disease. Plants, on the other hand, evolved disease resistance genes that recognize some of the effectors or avirulence (Avr) proteins. Both, identification of the Avr proteins and understanding of the mechanisms of action of other effectors, are important areas of research in the molecular plant-pathogen interactions field as this knowledge is critical for the development of new effective pathogen control measures. To enable functional analysis of the effectors it is desirable to be able to overexpress them readily in the host plants. Here we describe detailed experimental protocols for transient effector overexpression in wheat and other monocots using binary Barley stripe mosaic virus (BSMV) and Foxtail mosaic virus (FoMV) derived vectors. This functional genomics tool, better known as VOX (Virus-mediated protein OvereXpression), is rapid and relatively simple and inexpensive.

KeywordsVOX; BSMV; FoMV; Effectors; Cereals; Wheat; Functional genomics
Page range2523 p 93–112
Year of Publication2022
Book titleEffector-triggered immunity
PublisherHumana Press Inc
SeriesMethods in Molecular Biology
ISSN1064-3745
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-0716-2449-4_7
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online28 Jun 2022

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