Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

A - Papers appearing in refereed journals

Hahn, F., Sanjurjo-Loures, L., Sparks, C. A., Kanyuka, K. and Nekrasov, V. 2021. Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties. Plants - Basel. 10 (7), p. 1481. https://doi.org/10.3390/plants10071481

AuthorsHahn, F., Sanjurjo-Loures, L., Sparks, C. A., Kanyuka, K. and Nekrasov, V.
Abstract

CRISPR/Cas technology has recently become the molecular tool of choice for gene function studies in plants as well as crop improvement. Wheat is a globally important staple crop with a well annotated genome and there is plenty of scope for improving its agriculturally important traits using genome editing technologies, such as CRISPR/Cas. As part of this study we targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-eIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert. Primary transgenic lines carrying CRISPR/Cas-induced indels were successfully generated for all targeted genes. While BAK1 is an important regulator of plant immunity and development, Ta-eIF4E and Ta-eIF(iso)4E act as susceptibility (S) factors required for plant viruses from the Potyviridae family to complete their life cycle. We anticipate the resultant homozygous tabak1-2 mutant lines will facilitate studies on the involvement of BAK1 in immune responses in wheat, while ta-eif4e and ta-eif(iso)4e mutant lines have the potential to become a source of resistance to wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV), both of which are important pathogens of wheat. As winter wheat varieties are generally less amenable to genetic transformation, the successful experimental methodology for transformation and genome editing in winter wheat presented in this study will be of interest to the research community working with this crop.

KeywordsCRISPR; Cas9; Plant; Genome editing; BAK1; elF4E; Wheat
Year of Publication2021
JournalPlants - Basel
Journal citation10 (7), p. 1481
Digital Object Identifier (DOI)https://doi.org/10.3390/plants10071481
Web address (URL)https://www.mdpi.com/2223-7747/10/7/1481
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
Publisher's version
Supplemental file
Output statusPublished
Publication dates
Online19 Jul 2021
Publication process dates
Accepted16 Jul 2021
PublisherMDPI
ISSN2223-7747

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