Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

A - Papers appearing in refereed journals

Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D. and Kamoun, S. 2017. Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Scientific Reports. 7 (1), p. 482. https://doi.org/10.1038/s41598-017-00578-x

AuthorsNekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D. and Kamoun, S.
Abstract

Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/Cas9 being a highly precise tool, as we did not detect off-target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.

KeywordsCRISPR; Cas9; MLO; GMO; Genome editing
Year of Publication2017
JournalScientific Reports
Journal citation7 (1), p. 482
Digital Object Identifier (DOI)https://doi.org/10.1038/s41598-017-00578-x
Web address (URL)https://www.nature.com/articles/s41598-017-00578-x
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
European Research Council
Publisher's version
Output statusPublished
Publication dates
Online28 Mar 2017
Publication process dates
Accepted22 Feb 2017
PublisherNature Publishing Group
ISSN2045-2322

Permalink - https://repository.rothamsted.ac.uk/item/8wy99/rapid-generation-of-a-transgene-free-powdery-mildew-resistant-tomato-by-genome-deletion

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