Plant U-box E3 ligases PUB20 and PUB21 negatively regulate pattern-triggered immunity in Arabidopsis

A - Papers appearing in refereed journals

Yi, S.Y., Nekrasov, V., Ichimura, K., Kang, S.Y. and Shirasu, K. 2024. Plant U-box E3 ligases PUB20 and PUB21 negatively regulate pattern-triggered immunity in Arabidopsis. Plant Molecular Biology. 114 (7), pp. 1-14. https://doi.org/10.1007/s11103-023-01409-6

AuthorsYi, S.Y., Nekrasov, V., Ichimura, K., Kang, S.Y. and Shirasu, K.
Abstract

Plant U-box proteins (PUBs) constitute a class of E3 ligases that are associated with various stress responses. Among the class IV PUBs featuring C-terminal Armadillo (ARM) repeats, PUB20 and PUB21 are closely related homologs. Here, we show that both PUB20 and PUB21 negatively regulate innate immunity in plants. Loss of PUB20 and PUB21 function leads to enhanced resistance to surface inoculation with the virulent bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). However, the resistance levels remain unaffected after infiltration inoculation, suggesting that PUB20 and PUB21 primarily function during the early defense stages. The enhanced resistance to Pst DC3000 in PUB mutant plants (pub20-1, pub21-1, and pub20-1/pub21-1) correlates with extensive flg22-triggered reactive oxygen production, strong MPK3 activation, and enhanced transcriptional activation of early immune response genes. Additionally, PUB mutant plants (except pub21-1) exhibit constitutive stomatal closure after Pst DC3000 inoculation, implying the significant role of PUB20 in stomatal immunity. Comparative analyses of flg22 responses between PUB mutants and wild-type plants reveals that the robust activation of the pattern-induced immune responses may enhance resistance against Pst DC3000. Notably, the hypersensitivity responses triggered by RPM1/avrRpm1 and RPS2/avrRpt2 are independent of PUB20 and PUB21. These results suggest that PUB20 and PUB21 knockout mutations affect bacterial invasion, likely during the early stages, acting as negative regulators of plant immunity.

KeywordsArabidopsis; E3 ligase; PUB20; PUB21; Pattern-triggered immunity
Year of Publication2024
JournalPlant Molecular Biology
Journal citation114 (7), pp. 1-14
Digital Object Identifier (DOI)https://doi.org/10.1007/s11103-023-01409-6
PubMed ID38265485
Web address (URL)https://link.springer.com/article/10.1007/s11103-023-01409-6#citeas
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online24 Jan 2024
Publication process dates
Accepted14 Dec 2023
PublisherSpringer
ISSN0167-4412

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