Identification of Leaf Promoters for Use in Transgenic Wheat

A - Papers appearing in refereed journals

Alotaibi, S. S., Sparks, C. A., Parry, M. A. J., Simkin, A. J. and Raines, C. A. 2018. Identification of Leaf Promoters for Use in Transgenic Wheat. Plants. 7 (2), p. 27.

AuthorsAlotaibi, S. S., Sparks, C. A., Parry, M. A. J., Simkin, A. J. and Raines, C. A.
Abstract

Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields.

KeywordsPromoter; Wheat; Tissue specific; Photosynthesis; Reporter gene; Yield
Year of Publication2018
JournalPlants
Journal citation7 (2), p. 27
Digital Object Identifier (DOI)doi:10.3390/plants7020027
PubMed ID29597282
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or code[20:20 Wheat] Maximising yield potential of wheat
Publisher's version
Output statusPublished
Publication dates
Online28 Mar 2018
Publication process dates
Accepted23 Mar 2018
PublisherMDPI
Copyright licenseCC BY
ISSN2223-7747

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