Genetic transformation of common wheat (Triticum aestivum L.) using biolistics
B - Book chapters etc edited externally
Sparks, C. A. and Doherty, A. 2020. Genetic transformation of common wheat (Triticum aestivum L.) using biolistics. in: Rustgi, S. and Luo, H. (ed.) Biolistic DNA Delivery in Plants New York Humana Press Inc.
| Authors | Sparks, C. A. and Doherty, A. |
|---|---|
| Editors | Rustgi, S. and Luo, H. |
| Abstract | The following protocol describes the genetic transformation of wheat using the BioRad PDS/1000-He particle delivery system. Immature embryos are isolated 12-16 days post anthesis, the embryonic axis is removed, and the immature scutella are pre-cultured for 1-2 days prior to particle bombardment. Gold particles are coated with plasmid DNA containing the gene(s) of interest plus a selectable marker gene, in this instance bar (bialaphos resistance), and are fired into the cells to deliver the DNA. Subsequent tissue culture and regeneration steps allow recovery of plantlets, assisted by the inclusion of PPT (phosphinothricin tripeptide), the active ingredient of glufosinate-ammonium containing herbicides, to help select transformants. This updated method introduces selection earlier in the regeneration process which provides a shortened protocol while maintaining high transformation efficiencies. |
| Keywords | Biolistics; Gene gun; Particle bombardment; Wheat; Genetic transformation; Immature embryo; Transgenic plants; DNA delivery; Tissue culture; Regeneration |
| Year of Publication | 2020 |
| Book title | Biolistic DNA Delivery in Plants |
| Publisher | Humana Press Inc |
| Place of publication | New York |
| Series | Methods in Molecular Biology, vol 2124 |
| ISBN | 978-1-0716-0355-0 |
| Digital Object Identifier (DOI) | https://doi.org/10.1007/978-1-0716-0356-7_12 |
| Funder | Biotechnology and Biological Sciences Research Council |
| Funder project or code | Designing Future Wheat (DFW) [ISPG] |
| Open access | Published as non-open access |
| Output status | Published |
| Publication dates | |
| Online | 11 Apr 2020 |
| Copyright license | Publisher copyright |
Related outputs
A high soluble-fibre allele in wheat encodes a defective cell wall peroxidase responsible for dimerization of ferulate moieties on arabinoxylan
A - Papers appearing in refereed journals
Mitchell, R. A. C., Oszvald, M., Pellny, T. K., Freeman, J., Halsey, K., Sparks, C. A., Huttly, A. K., Specel, S., Leverington-Waite, M., Griffiths, S., Shewry, P. R. and Lovegrove, A. 2023. A high soluble-fibre allele in wheat encodes a defective cell wall peroxidase responsible for dimerization of ferulate moieties on arabinoxylan. bioRxiv. https://doi.org/10.1101/2023.03.08.531735
Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants
A - Papers appearing in refereed journals
Hyde, L., Osman, K., Winfield, M., Sanchez-Moran, E., Higgins, J. D., Henderson, I. R., Sparks, C. A., Franklin, F. C. H. and Edwards, K. 2022. Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants. Plant Biotechnology Journal. 2022, pp. 1-4. https://doi.org/10.1111/pbi.13961
Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties
A - Papers appearing in refereed journals
Hahn, F., Sanjurjo-Loures, L., Sparks, C. A., Kanyuka, K. and Nekrasov, V. 2021. Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties. Plants - Basel. 10 (7), p. 1481. https://doi.org/10.3390/plants10071481
Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2
A - Papers appearing in refereed journals
Raffan, S., Sparks, C. A., Huttly, A. K., Hyde, L., Martignago, D., Mead, A., Hanley, S. J., Wilkinson, P., Barker, G., Edwards, K. J., Curtis, T. Y., Usher, S. L., Kosik, O. and Halford, N. G. 2021. Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2 . Plant Biotechnology Journal. 19 (8), pp. 1602-1613. https://doi.org/10.1111/pbi.13573
Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
A - Papers appearing in refereed journals
Wan, Y., Wang, Y., Shi, Z., Rentsch, D., Ward, J. L., Hassall, K. L., Sparks, C. A., Huttly, A. K., Buchner, P. H., Powers, S. J., Shewry, P. R. and Hawkesford, M. J. 2021. Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain. PLOS ONE. 16 (2), p. e0246763. https://doi.org/10.1371/journal.pone.0246763
CRISPR-Cas9 multiplex editing of the α-amylase/trypsin inhibitor genes to reduce allergen proteins in durum wheat
A - Papers appearing in refereed journals
Camerlengo, F., Frittelli, A., Sparks, C. A., Doherty, A., Martignago, D., Larre, C., Lupi, R., Sestili, F. and Masci, S. 2020. CRISPR-Cas9 multiplex editing of the α-amylase/trypsin inhibitor genes to reduce allergen proteins in durum wheat. Frontiers in Sustainable Food Systems. https://doi.org/10.3389/fsufs.2020.00104
Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat
A - Papers appearing in refereed journals
Lobo, A. K. M., Orr, D., Gutierrez, M. O., Andralojc, P. J., Sparks, C. A., Afan, M., Parry, J., Parry, M. A. J. and Carmo-Silva, E. 2019. Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat. Plant Physiology. 181 (October), pp. 471-479. https://doi.org/10.1104/pp.19.00693
Use of the pre-assembled ribonucleoprotein (RNP) complex for gene editing of wheat and poplar
Conference poster
Sparks, C. A., Cerezo-Medina, S., Raffan, S. and Hahn, F. 2019. Use of the pre-assembled ribonucleoprotein (RNP) complex for gene editing of wheat and poplar. The 7th Plant Genomics and Gene Editing Congress. Rotterdam, The Netherlands 21 May 2019
Silencing an essential gene involved in infestation and digestion in grain aphid through plant‐mediated RNA interference generates aphid‐resistant wheat plants
A - Papers appearing in refereed journals
Sun, Y., Sparks, C. A., Jones, H., Riley, M., Francis, F., Du, W. and Xia, L. 2019. Silencing an essential gene involved in infestation and digestion in grain aphid through plant‐mediated RNA interference generates aphid‐resistant wheat plants. Plant Biotechnology Journal. 17 (5), pp. 852-854. https://doi.org/10.1111/pbi.13067
Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici
A - Papers appearing in refereed journals
Kettles, G. J., Bayon, C., Sparks, C. A., Canning, G., Kanyuka, K. and Rudd, J. J. 2018. Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici. New Phytologist. 217 (1), pp. 320-331. https://doi.org/10.1111/nph.14786
Identification of Leaf Promoters for Use in Transgenic Wheat
A - Papers appearing in refereed journals
Alotaibi, S. S., Sparks, C. A., Parry, M. A. J., Simkin, A. J. and Raines, C. A. 2018. Identification of Leaf Promoters for Use in Transgenic Wheat. Plants - Basel. 7 (2), p. 27. https://doi.org/10.3390/plants7020027
Host-induced gene silencing for the control of the Fusarium Head Blight in wheat fields
C2 - Non-edited contributions to conferences
Kanyuka, K., Machado, A., Urban, M., Lee, W. S., Brown, N. J., King, R. A., Lau, E. Y., Sparks, C. A., Bonato, A. L. V., Tibola, C. S., Martins, N. F., Aragao, F. J., West, J., Fernandes, J. M. C. and Hammond-Kosack, K. E. 2017. Host-induced gene silencing for the control of the Fusarium Head Blight in wheat fields. 1st COST iPlanta Conferernce; "Creating the plant RNAi research network CA15223", La Sapienza - CNR Headquarter , Rome, Italy, 15-17 February 2017.
Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions
A - Papers appearing in refereed journals
Driever, S. M., Simkin, A. J., Alotaibi, S., Fisk, S. J., Madgwick, P. J., Sparks, C. A., Jones, H. D., Lawson, T., Parry, M. A. J. and Raines, C. A. 2017. Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions. Philosophical Transactions of the Royal Society B-Biological Sciences. 372 (1730), pp. 1-10. https://doi.org/10.1098/rstb.2016.0384
Use of a modified CRISPR/Cas9 construct to simultaneously mutagenize multiple genes in hexaploid wheat
C1 - Edited contributions to conferences/learned societies
Winfield, M., Allen, S., Cartlidge, L., Sparks, C. A., Jones, H., Huttly, A. K., Martignago, D., Hawkesford, M. J. and Edwards, K. 2017. Use of a modified CRISPR/Cas9 construct to simultaneously mutagenize multiple genes in hexaploid wheat. MonoGram 2017, 4-6 April, University of Bristol. Bristol, University of Bristol.
Opportunities for research via wheat transformation
C2 - Non-edited contributions to conferences
Sparks, C. A., Martignago, D., Huttly, A. K., Raffan, S., Doherty, A. and Edwards, K. 2017. Opportunities for research via wheat transformation. The 5th Plant Genomics and Gene Editing Congress, Amsterdam, 16–17 March 2017. Societas Physiologiae Plantarum Scandinavica.
Wheat biotechnology: recent developments and future trends
B - Book chapters etc edited externally
Sparks, C. A. 2016. Wheat biotechnology: recent developments and future trends. in: Jones, H. D. (ed.) Biotechnology of major cereals Chapter 6 CABI International, Wallingford, Oxon (CABI). pp. 88-103
The first crop plant genetically engineered to release an insect pheromone for defence
A - Papers appearing in refereed journals
Bruce, T. J. A., Aradottir, G. I., Smart, L. E., Martin, J. L., Caulfield, J. C., Doherty, A., Sparks, C. A., Woodcock, C. M., Birkett, M. A., Napier, J. A., Jones, H. D. and Pickett, J. A. 2015. The first crop plant genetically engineered to release an insect pheromone for defence. Scientific Reports. 5, p. 11183. https://doi.org/10.1038/srep11183
Genetic transformation of wheat via particle bombardment
B - Book chapters etc edited externally
Sparks, C. A. and Jones, H. D. 2014. Genetic transformation of wheat via particle bombardment. in: Henry, R. J. and Furtado, A. (ed.) Cereal genomics: methods and protocols. Methods in Molecular Biology, vol 1099 Springer, New York. pp. 201-218
Genetic transformation of wheat via Agrobacterium-mediated DNA delivery
B - Book chapters etc edited externally
Sparks, C. A., Doherty, A. and Jones, H. D. 2014. Genetic transformation of wheat via Agrobacterium-mediated DNA delivery. in: Henry, R. J. and Furtado, A. (ed.) Cereal genomics: methods and protocols. Methods in Molecular Biology, vol 1099 Springer, New York. pp. 235-250
Temporal and spatial transgene expression using a heat-shock promoter in transgenic wheat
C1 - Edited contributions to conferences/learned societies
Freeman, J., Sparks, C. A., West, J. S., Shewry, P. R. and Jones, H. D. 2011. Temporal and spatial transgene expression using a heat-shock promoter in transgenic wheat. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 85-91
Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene
C1 - Edited contributions to conferences/learned societies
Bruce, T. J. A., Smart, L. E., Martin, J. L., Doherty, A., Jones, H. D., Napier, J. A. and Pickett, J. A. 2011. Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 24
Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene
C1 - Edited contributions to conferences/learned societies
Bruce, T. J. A., Smart, L. E., Aradottir, G. I., Martin, J. L., Doherty, A., Jones, H. D., Napier, J. A. and Pickett, J. A. 2011. Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 112
Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene
C2 - Non-edited contributions to conferences
Bruce, T. J. A., Smart, L. E., Aradottir, G. I., Martin, J. L., Doherty, A., Jones, H. D., Napier, J. A. and Pickett, J. A. 2011. Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene. Abstracts Ento' 11 Symposium on Chemical Ecology: Reception, Detection and Deception, Chatham, 7-9 September 2011 . pp. 54
Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat
A - Papers appearing in refereed journals
Pearce, S., Saville, R., Vaughan, S. P., Chandler, P. M., Wilhelm, E. P., Sparks, C. A., Al-Kaff, N., Korolev, A., Boulton, M. I., Phillips, A. L., Hedden, P., Nicholson, P. and Thomas, S. G. 2011. Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat. Plant Physiology. 157, pp. 1820-1831. https://doi.org/10.1104/pp.111.183657
Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat
A - Papers appearing in refereed journals
Freeman, J., Sparks, C. A., West, J., Shewry, P. R. and Jones, H. D. 2011. Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotechnology Journal. 9 (7), pp. 788-796. https://doi.org/10.1111/j.1467-7652.2011.00588.x
Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging
C1 - Edited contributions to conferences/learned societies
Tosi, P., Gritsch, C., Freeman, J., Sparks, C. A., Jones, H. D., Funatsuki, W., Niwa, K. and Shewry, P. R. 2010. Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging. Branlard, G. (ed.) Gluten Proteins 2009: Proceedings 10th International Gluten Workshop, Clermont-Ferrand, 7-9 September 2009 . Institut National de la Recherche Agronomique (INRA), Paris. pp. 17-20
Dough properties of transgenic wheat transformed with Cys-mutated low-molecular-weight glutenin genes
C1 - Edited contributions to conferences/learned societies
Funatsuki, W., Tosi, P., Ito, M., Jones, H. D., Sparks, C. A., Riley, A., Nishio, Z., Tadashi, T., Yamauchi, H. and Shewry, P. R. 2010. Dough properties of transgenic wheat transformed with Cys-mutated low-molecular-weight glutenin genes. Branlard, G. (ed.) Gluten Proteins 2009: Proceedings of the 10th International Gluten Workshop, Clermont-Ferrand, 7-9 September 2009 . Institut National de la Recherche Agronomique (INRA), Paris. pp. 175-179
Increasing the amylose content of durum wheat through silencing of the SBE11a genes
A - Papers appearing in refereed journals
Sestili, F., Janni, M., Doherty, A., Botticella, E., D'ovidio, R., Masci, S., Jones, H. D. and Lafiandra, D. 2010. Increasing the amylose content of durum wheat through silencing of the SBE11a genes. BMC Plant Biology. 10, p. 144 (12pp). https://doi.org/10.1186/1471-2229-10-144
Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat
A - Papers appearing in refereed journals
Nemeth, C., Freeman, J., Jones, H. D., Sparks, C. A., Pellny, T. K., Wilkinson, M. D., Dunwell, J., Andersson, A. A. M., Aman, P., Guillon, F., Saulnier, L., Mitchell, R. A. C. and Shewry, P. R. 2010. Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat. Plant Physiology. 152, pp. 1209-1218. https://doi.org/10.1104/pp.109.151712
Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging
C2 - Non-edited contributions to conferences
Tosi, P., Gritsch, C., Freeman, J., Sparks, C. A., Jones, H. D., Funatsuki, W., Niwa, K. and Shewry, P. R. 2009. Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging. Abstracts Xth International Gluten Workshop, Clermont Ferrand, 7-9 September 2009 .
Stable transformation of plants
B - Book chapters etc edited externally
Jones, H. D. and Sparks, C. A. 2009. Stable transformation of plants. in: Gustafson, J. P., Somers, D. J. and Langridge, P. (ed.) Plant genomics. (Methods in Molecular Biology vol 513) Humana Press, Totowa, New Jersey. pp. 111-130
Transient transformation of plants
B - Book chapters etc edited externally
Jones, H. D., Doherty, A. and Sparks, C. A. 2009. Transient transformation of plants. in: Gustafson, J. P., Somers, D. J. and Langridge, P. (ed.) Plant genomics. (Methods in Molecular Biology vol 513) Humana Press, Totowa, New Jersey. pp. 131-152
Transgenic manipulation of wheat quality
B - Book chapters etc edited externally
Jones, H. D. and Sparks, C. A. 2009. Transgenic manipulation of wheat quality. in: Shewry, P. R. and Khan, K. (ed.) Wheat: chemistry and technology, 4th ed American Association of Cereal Chemists (AACC), St Paul, MN. pp. 437-451
A transgenic approach to understanding gene expression in cereals
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Freeman, J. and Sparks, C. A. 2008. A transgenic approach to understanding gene expression in cereals. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 427-430
Agrobacterium- mediated transformation of bread and durum wheat using freshly isolated immature embryos
B - Book chapters etc edited externally
Wu, H. and Doherty, A. 2008. Agrobacterium- mediated transformation of bread and durum wheat using freshly isolated immature embryos. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 93-103
Biolistics transformation of wheat
B - Book chapters etc edited externally
Sparks, C. A. 2008. Biolistics transformation of wheat. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 71-92
Selection of transformed plants
B - Book chapters etc edited externally
Sparks, C. A. 2008. Selection of transformed plants. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 23-37
Promoter sequences for defining transgene expression
B - Book chapters etc edited externally
Sparks, C. A. 2008. Promoter sequences for defining transgene expression. in: Shewry, P. R. and Jones, H. D. (ed.) In %Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 171-184
Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development
A - Papers appearing in refereed journals
Van Herpen, T. W. J. M., Riley, M., Sparks, C. A., Jones, H. D., Gritsch, C., Dekking, E. H., Hamer, R. J., Bosch, D., Salentijn, E. M. J., Smulders, M. J. M., Shewry, P. R. and Gilissen, L. J. W. J. 2008. Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development. Annals of Botany. 102, pp. 331-342. https://doi.org/10.1093/aob/mcn114
Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes
A - Papers appearing in refereed journals
Wu, H., Doherty, A. and Jones, H. D. 2008. Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes. Transgenic Research. 17, pp. 425-436. https://doi.org/10.1007/s11248-007-9116-9
The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations
A - Papers appearing in refereed journals
Pastori, G. M., Huttly, A. K., West, J., Sparks, C. A., Pieters, A., Luna, C. M., Jones, H. D. and Foyer, C. H. 2007. The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations. Functional Plant Biology. 34 (9), pp. 835-843. https://doi.org/10.1071/FP07112
High throughput Agrobacterium transformation of wheat: a tool for functional genomics
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wilkinson, M. D., Doherty, A. and Wu, H. 2007. High throughput Agrobacterium transformation of wheat: a tool for functional genomics. Buck, H. T., Salomon, N. and Nisi, J. E. (ed.) Wheat Production in Stressed Environments: Proceedings 7th International Wheat Conference, Mar del Plata, Argentina, 27 November-2 December 2005 . Springer, Dordrecht. pp. 693-699
Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength
C2 - Non-edited contributions to conferences
Maruyama-Funatsuki, W., Tosi, P., Jones, H. D., Sparks, C. A., Riley, A., Yamauchi, H. and Shewry, P. R. 2007. Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength.
Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins
C1 - Edited contributions to conferences/learned societies
Shewry, P. R., Tosi, P., Jones, H. D., Sparks, C. A., Gritsch, C., Funatsuki, W., Niwa, K., Huttly, A. K., Napier, J. A., D'ovidio, R. and Freeman, J. 2007. Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins. Lookhart, G. L. and Ng, P. W. K. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 112-116
Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation
A - Papers appearing in refereed journals
Wu, H., Sparks, C. A. and Jones, H. D. 2006. Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation. Molecular Breeding. 18, pp. 195-208. https://doi.org/10.1007/s11032-006-9027-0
Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat
A - Papers appearing in refereed journals
Jones, H. D., Doherty, A. and Wu, H. 2005. Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat. Plant Methods. 1:5. https://doi.org/10.1186/1746-4811-1-5
Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25
A - Papers appearing in refereed journals
Howarth, J. R., Jacquet, J. N., Doherty, A., Jones, H. D. and Cannell, M. E. 2005. Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25. Annals of Applied Biology - AAB. 146 (3), pp. 311-320. https://doi.org/10.1111/j.1744-7348.2005.040121.x
Transformation of wheat by biolistics
B - Book chapters etc edited externally
Sparks, C. A. and Jones, H. D. 2004. Transformation of wheat by biolistics. in: Curtis, I. S. (ed.) Transgenic crops of the world: essential protocols Kluwer Academic Publishers, Dordrecht, Netherlands. pp. 19-33
Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)
A - Papers appearing in refereed journals
Mendoza De Gyves, E., Sparks, C. A., Sayanova, O. V., Lazzeri, P. A., Napier, J. A. and Jones, H. D. 2004. Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.). Plant Biotechnology Journal. 2 (4), pp. 351-357. https://doi.org/10.1111/j.1467-7652.2004.00079.x
The potential of biotechnology to produce novel gluten phenotypes
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Sparks, C. A., Doherty, A., Cannell, M., Goodwin, J., Pastori, G. M. and Shewry, P. R. 2004. The potential of biotechnology to produce novel gluten phenotypes. D'ovidio, R., Lafiandra, D. and Masci, S. (ed.) Gluten Proteins. Proceedings of the 8th Gluten Workshop, Viterbo, 8-10 September 2003 . Royal Society of Chemistry (RSC), Cambridge. pp. 14-17
Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat
C2 - Non-edited contributions to conferences
Doherty, A., West, J. M., Sparks, C. A., Stone, M. C., Cannell, M. and Jones, H. D. 2003. Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 .
Transgenic approaches to study quality traits in cereals
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Sparks, C. A. and Shewry, P. R. 2003. Transgenic approaches to study quality traits in cereals. Proceedings BCPC International Congress - Crop Science and Technology, Glasgow, 10-12 November 2003 . pp. 133-140
Promoters for driving transgene expression in wheat
C1 - Edited contributions to conferences/learned societies
Sparks, C. A., Collins, R. and Jones, H. D. 2003. Promoters for driving transgene expression in wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 .
Optimisation factors for Agrobacterium -mediated genetic transformation of wheat (cv Florida)
C1 - Edited contributions to conferences/learned societies
Wu, H., Sparks, C. A. and Jones, H. D. 2003. Optimisation factors for Agrobacterium -mediated genetic transformation of wheat (cv Florida). Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 . pp. 181-184
Agrobacterium mediated transformation of wheat: progress and application
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Patil, M. S., Rubio, S. and Sparks, C. A. 2003. Agrobacterium mediated transformation of wheat: progress and application. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 . pp. 914-916
Factors influencing successful Agrobacterium -mediated genetic transformation of wheat
A - Papers appearing in refereed journals
Wu, H., Sparks, C. A., Amoah, B. and Jones, H. D. 2003. Factors influencing successful Agrobacterium -mediated genetic transformation of wheat. Plant Cell Reports. 21, pp. 659-668.
Age-dependent transformation frequency in elite wheat varieties
A - Papers appearing in refereed journals
Pastori, G. M., Wilkinson, M. D., Steele, S. H., Sparks, C. A., Jones, H. D. and Parry, M. A. J. 2001. Age-dependent transformation frequency in elite wheat varieties. Journal of Experimental Botany. 52, pp. 857-863.
High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron
A - Papers appearing in refereed journals
Mendoza De Gyves, E., Sparks, C. A., Fieldsend, A. F., Lazzeri, P. A. and Jones, H. D. 2001. High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron. Annals of Applied Biology - AAB. 138 (3), pp. 329-332.
Factors influencing Agrobacterium -mediated transient expression of uid A in wheat inflorescence tissue
A - Papers appearing in refereed journals
Amoah, B. K., Wu, H., Sparks, C. A. and Jones, H. D. 2001. Factors influencing Agrobacterium -mediated transient expression of uid A in wheat inflorescence tissue. Journal of Experimental Botany. 52, pp. 1135-1142.
Potential for manipulating carbon metabolism in wheat
A - Papers appearing in refereed journals
Sparks, C. A., Castleden, C. K., West, J., Habash, D. Z., Madgwick, P. J., Paul, M. J., Noctor, G., Harrison, J., Wu, R., Wilkinson, J., Quick, W. P., Parry, M. A. J., Foyer, C. H. and Miflin, B. J. 2001. Potential for manipulating carbon metabolism in wheat. Annals of Applied Biology - AAB. 138 (1), pp. 33-45. https://doi.org/10.1111/j.1744-7348.2001.tb00083.x
Overexpression of sucrose-phosphate synthase in wheat
C2 - Non-edited contributions to conferences
Castleden, C. K., Sparks, C. A., West, J. M., Quick, W. P. and Foyer, C. H. 1999. Overexpression of sucrose-phosphate synthase in wheat. Abstracts International Conference on Assimilate Transport and Partitioning, Newcastle NSW, 15-20 August 1999 . pp. 95
A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability
A - Papers appearing in refereed journals
Cannell, M. E., Doherty, A., Lazzeri, P. A. and Barcelo, P. 1999. A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability. Theoretical and Applied Genetics. 99, pp. 772-784.
Current status of transformationin bread and durum wheats and modifications of gluten quality
C1 - Edited contributions to conferences/learned societies
He, G. Y., Rooke, L., Cannell, M., Rasco-Gaunt, S., Sparks, C. A., Lamacchia, C., Bekes, F., Tatham, A. S., Barcelo, P., Shewry, P. R. and Lazzeri, P. A. 1998. Current status of transformationin bread and durum wheats and modifications of gluten quality.
Transient expression of CAT in wheat protoplasts.
G - Articles in popular magazines and other technical publications
Jones, M. G. K., De Both, M. T. J., Wu, Q. S., Sparks, C. A., Maddock, S. E. and Gleddie, S. 1986. Transient expression of CAT in wheat protoplasts.
Permalink - https://repository.rothamsted.ac.uk/item/8wxx4/genetic-transformation-of-common-wheat-triticum-aestivum-l-using-biolistics