Expanding knowledge of the Rubisco kinetics variability in plant species: environmental and evolutionary trends

A - Papers appearing in refereed journals

Galmes, J., Kapralov, M. V., Andralojc, P. J., Conesa, M. A., Keys, A. J., Parry, M. A. J. and Flexas, J. 2014. Expanding knowledge of the Rubisco kinetics variability in plant species: environmental and evolutionary trends. Plant, Cell and Environment. 37 (9), pp. 1989-2001. https://doi.org/10.1111/pce.12335

AuthorsGalmes, J., Kapralov, M. V., Andralojc, P. J., Conesa, M. A., Keys, A. J., Parry, M. A. J. and Flexas, J.
Abstract

The present study characterizes the kinetic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from 28 terrestrial plant species, representing different phylogenetic lineages, environmental adaptations and photosynthetic mechanisms. Our findings confirm that past atmospheric CO2/O-2 ratio changes and present environmental pressures have influenced Rubisco kinetics. One evolutionary adaptation to a decreasing atmospheric CO2/O-2 ratio has been an increase in the affinity of Rubisco for CO2 (K-c falling), and a consequent decrease in the velocity of carboxylation (k(cat)(c)), which in turn has been ameliorated by an increase in the proportion of leaf protein accounted by Rubisco. The trade-off between K-c and k(cat)(c) was not universal among the species studied and deviations from this relationship occur in extant forms of Rubisco. In species adapted to particular environments, including carnivorous plants, crassulacean acid metabolism species and C-3 plants from aquatic and arid habitats, Rubisco has evolved towards increased efficiency, as demonstrated by a higher k(cat)(c)/K-c ratio. This variability in kinetics was related to the amino acid sequence of the Rubisco large subunit. Phylogenetic analysis identified 13 residues under positive selection during evolution towards specific Rubisco kinetic parameters. This crucial information provides candidate amino acid replacements, which could be implemented to optimize crop photosynthesis under a range of environmental conditions.

KeywordsPlant Sciences
Year of Publication2014
JournalPlant, Cell and Environment
Journal citation37 (9), pp. 1989-2001
Digital Object Identifier (DOI)https://doi.org/10.1111/pce.12335
PubMed ID24689692
Open accessPublished as bronze (free) open access
FunderBiotechnology and Biological Sciences Research Council
Plan Nacional, Spain
Funder project or codeWheat
[20:20 Wheat] Maximising yield potential of wheat
Publisher's version
PublisherWiley
Wiley-Blackwell
Grant IDAGL2009-07999
BFU2011-23294
BB/J/00426X/1
BB/I002545/1
BB/I017372/1
ISSN0140-7791

Permalink - https://repository.rothamsted.ac.uk/item/8qy59/expanding-knowledge-of-the-rubisco-kinetics-variability-in-plant-species-environmental-and-evolutionary-trends

15 total views
18 total downloads
1 views this month
0 downloads this month
Download files as zip