Regulation of Rubisco by inhibitors in the light

A - Papers appearing in refereed journals

Parry, M. A. J., Andralojc, P. J., Parmar, S., Keys, A. J., Habash, D., Paul, M. J., Alred, R., Quick, W. P. and Servaites, J. C. 1997. Regulation of Rubisco by inhibitors in the light. Plant, Cell & Environment. 20 (4), pp. 528-534.

AuthorsParry, M. A. J., Andralojc, P. J., Parmar, S., Keys, A. J., Habash, D., Paul, M. J., Alred, R., Quick, W. P. and Servaites, J. C.
Abstract

2-carboxy-D-arabinitol-1-phosphate (CA1P) bound to Rubisco either in leaf extracts or after purification can be displaced by SO42- ions, Thus, treatment of leaf extracts with a buffer containing 200 mol m(-3) SO42- displaces any bound CA1P and enables measurement of maximum carboxylation potential, In tobacco leaves, the activity following treatment with SO42- ions ('maximal activity') is greater than the total Rubisco activity, The ratio of the two activities altered in a dynamic way with fluctuations in irradiance, Even in species which do not produce significant amounts of CA1P, the maximal activity greatly exceeded the total activity, Anion exchange separation of components in acid extracts confirmed the absence of CA1P in tobacco leaves harvested above an irradiance of 300 mu mol quanta m(-2) s(-1), but the presence of another inhibitor of Rubisco, These results are consistent with the regulation of Rubisco activity by inhibitors other than CA1P which, like CA1P, can be displaced by SO42- ions.

KeywordsPlant Sciences
Year of Publication1997
JournalPlant, Cell & Environment
Journal citation20 (4), pp. 528-534
Digital Object Identifier (DOI)doi:10.1046/j.1365-3040.1997.d01-85.x
Open accessPublished as non-open access
Funder project or code116
214
414
ISSN01407791
PublisherWiley

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