Sugar sensing responses to low and high light in leaves of the C4 model grass Setaria viridis

A - Papers appearing in refereed journals

Henry, C., Watson-Lazowski, A., Oszvald, M., Griffiths, C. A., Paul, M. J., Furbank, R. T. and Ghannoum, O. 2019. Sugar sensing responses to low and high light in leaves of the C4 model grass Setaria viridis. Journal of Experimental Botany.

AuthorsHenry, C., Watson-Lazowski, A., Oszvald, M., Griffiths, C. A., Paul, M. J., Furbank, R. T. and Ghannoum, O.
Abstract

Although sugar regulate photosynthesis, the signalling pathways underlying this process remain elusive, especially for C4 crops. To address this knowledge gap and identify potential candidate genes, we treated Setaria viridis (C4 model) plants acclimated to medium light intensity (ML, 500 µmol m-2 s-1) with low (LL, 50 µmol m-2 s-1) or high (HL, 1000 µmol m-2 s-1) light for 4 days and observed the consequences on carbon metabolism and the transcriptome of source leaves. LL impaired photosynthesis and reduced leaf content of signalling sugars (glucose, sucrose and trehalose-6-phosphate). Contrastingly, HL strongly induced sugar accumulation without repressing photosynthesis. LL more profoundly impacted leaf transcriptome, including photosynthetic genes. LL and HL contrastingly altered the expression of HXK and SnRK1 sugar sensors and trehalose pathway genes. The expression of key target genes of HXK and SnRK1 were affected by LL and sugar depletion, while surprisingly HL and strong sugar accumulation only slightly repressed the SnRK1 signalling pathway. In conclusion, we demonstrate that LL profoundly impacted photosynthesis and the transcriptome of S. viridis source leaves, while HL altered sugar levels more than LL. We also present the first evidence that sugar signalling pathways in C4 source leaves may respond to light intensity and sugar accumulation differently to C3 source leaves

KeywordsC4 photosynthesis; Glucose; Hexokinase (HXK); Setaria viridis; Sucrose; Sugar signalling; Sucrose-non fermenting 1 (Snf1) related protein kinase 1 (SnRK1); Target of rapamycin (TOR); Trehalose-6-phosphate
Year of Publication2019
JournalJournal of Experimental Botany
Digital Object Identifier (DOI)doi:10.1093/jxb/erz495
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat - WP1 - Increased efficiency and sustainability
Accepted author manuscript
Output statusPublished
Publication dates
Online02 Nov 2019
Publication process dates
Accepted11 Oct 2019
PublisherOxford University Press (OUP)
ISSN0022-0957

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