Analysis of small RNA silencing in Zymoseptoria tritici – wheat interactions

A - Papers appearing in refereed journals

Kettles, G. J., Hofinger, B. J., Hu, P., Bayon, C., Rudd, J. J., Balmer, D., Courbot, M., Hammond-Kosack, K. E., Scalliet, G. and Kanyuka, K. 2018. Analysis of small RNA silencing in Zymoseptoria tritici – wheat interactions. bioRxiv.

AuthorsKettles, G. J., Hofinger, B. J., Hu, P., Bayon, C., Rudd, J. J., Balmer, D., Courbot, M., Hammond-Kosack, K. E., Scalliet, G. and Kanyuka, K.
Abstract

Cross-kingdom small RNA (sRNA) silencing has recently emerged as a mechanism facilitating fungal colonization and disease development. Here we characterized RNAi pathways in Zymoseptoria tritici, a major fungal pathogen of wheat, and assessed their contribution to pathogenesis. Computational analysis of fungal sRNA and host mRNA sequencing datasets was used to define the global sRNA populations in Z. tritici and predict their mRNA targets in wheat. 389 in planta-induced sRNA loci were identified. sRNAs generated from some of these loci were predicted to target wheat mRNAs including those potentially involved in pathogen defense. However, molecular approaches failed to validate targeting of selected wheat mRNAs by fungal sRNAs. Mutant strains of Z. tritici carrying deletions of genes encoding key components of RNAi such as Dicer-like (DCL) and Argounate (AGO) proteins were generated, and virulence bioassays suggested that these are dispensable for full infection of wheat. Nonetheless, our results did suggest the existence of non-canonical DCL-independent pathway(s) for sRNA biogenesis in Z. tritici. dsRNA targeting essential fungal genes applied in vitro or generated from an RNA virus vector in planta in a procedure known as HIGS (Host-Induced Gene Silencing) was ineffective in preventing Z. tritici growth or disease. We also demonstrated that Z. tritici is incapable of dsRNA uptake. Collectively, our data suggest that RNAi approaches for gene function analyses in this fungal species and potentially also as a control measure may not be as effective as has been demonstrated for some other plant pathogenic fungi.

KeywordsCross--Kingdom RNAi; RNA interference (RNAi); small RNA (sRNA); Host-induced gene silencing (HIGS); Septoria tritici blotch; Zymoseptoria tritici; Triticum aestivum; effectors
Year of Publication2018
JournalbioRxiv
Digital Object Identifier (DOI)doi:10.1101/501650
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
[20:20 Wheat] Maximising yield potential of wheat
Publisher's version
Output statusPublished
Publication dates
Print19 Dec 2018
Publication process dates
Accepted20 Dec 2018
PublisherFrontiers Media SA
Public Library of Science (PLOS)
ISSN1664-462X
Copyright licenseCC BY

Permalink - https://repository.rothamsted.ac.uk/item/8w8q9/analysis-of-small-rna-silencing-in-zymoseptoria-tritici-wheat-interactions

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