Phenotypic and genetic analysis of the Triticum monococcum-Mycosphaerella graminicola interaction

A - Papers appearing in refereed journals

Jing, H-C., Lovell, D. J., Gutteridge, R. J., Jenk, D., Kornyukhin, D., Mitrofanova, O., Kema, G. H. J. and Hammond-Kosack, K. E. 2008. Phenotypic and genetic analysis of the Triticum monococcum-Mycosphaerella graminicola interaction. New Phytologist. 179, pp. 1121-1132.

AuthorsJing, H-C., Lovell, D. J., Gutteridge, R. J., Jenk, D., Kornyukhin, D., Mitrofanova, O., Kema, G. H. J. and Hammond-Kosack, K. E.
Abstract

Here, the aim was to understand the cellular and genetic basis of the Triticum monococcum–Mycosphaerella graminicola interaction.

Testing for 5 yr under UK field conditions revealed that all 24 T. monococcum accessions exposed to a high level of natural inocula were fully resistant to M. graminicola. When the accessions were individually inoculated in the glasshouse using an attached leaf seeding assay and nine previously characterized M. graminicola isolates, fungal sporulation was observed in only three of the 216 interactions examined. Microscopic analyses revealed that M. graminicola infection was arrested at four different stages post‐stomatal entry. When the inoculated leaves were detached 30 d post inoculation and incubated at 100% humidity, abundant asexual sporulation occurred within 5 d in a further 61 interactions.

An F2 mapping population generated from a cross between T. monococcum accession MDR002 (susceptible) and MDR043 (resistant) was inoculated with the M. graminicola isolate IPO323. Both resistance and in planta fungal growth were found to be controlled by a single genetic locus designated as TmStb1 which was linked to the microsatellite locus Xbarc174 on chromosome 7Am.

Exploitation of T. monococcum may provide new sources of resistance to septoria tritici blotch disease.

Year of Publication2008
JournalNew Phytologist
Journal citation179, pp. 1121-1132
Digital Object Identifier (DOI)doi:10.1111/j.1469-8137.2008.02526.x
Open accessPublished as bronze (free) open access
FunderDepartment of Environment, Food and Rural Affairs
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
Project: 8645
Pathogenicity of non-biotrophic fungi infecting cereals
The Defra wheat genetic improvement network (WGIN) [2003-2009]
Epidemiology of ear blight and biology of toxigenic Fusarium species and related pathogenic fungi in cereal crops
Optimising the performance and benefits of take-all chemicals
The Defra wheat genetic improvement network (WGIN) [2003-2009]
Publisher's version
Output statusPublished
Publication dates
Online06 Aug 2008
Publication process dates
Accepted23 Apr 2008
Copyright licensePublisher copyright
PublisherWiley
ISSN0028-646X

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