A - Papers appearing in refereed journals
Chancellor, T., Smith, D. P., Chen, W., Clark, S. J., Venter, E., Halsey, K., Carrera, E., Mcmillan, V. E., Canning, G., Armer, V., Hammond-Kosack, K. E. and Palma-Guerrero, J. 2024. A fungal endophyte induces local cell-wall mediated resistance in wheat roots against take-all disease. Frontiers in Plant Science. 15. https://doi.org/10.3389/fpls.2024.1444271
Authors | Chancellor, T., Smith, D. P., Chen, W., Clark, S. J., Venter, E., Halsey, K., Carrera, E., Mcmillan, V. E., Canning, G., Armer, V., Hammond-Kosack, K. E. and Palma-Guerrero, J. |
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Abstract | Take-all disease, caused by the ascomycete fungus Gaeumannomyces tritici, is one of the most important root diseases of wheat worldwide. The fungus invades the roots and destroys the vascular tissue, hindering the uptake of water and nutrients. Closely related non-pathogenic species in the Magnaporthaceae family, such as Gaeumannomyces hyphopodioides, occur naturally in arable and grassland soils and have previously been reported to reduce take-all disease in field studies. However, the mechanism of take-all protection has remained unknown. Here, we characterise the root infection biologies of G. tritici and G. hyphopodioides in wheat. We investigate the ultrastructure of previously described “subepidermal vesicles” (SEVs), produced in wheat roots by non-pathogenic G. hyphopodioides, but not by pathogenic G. tritici. We show that G. hyphopodioides SEVs share key characteristics of fungal resting structures; containing a greater number of putative lipid bodies and a significantly thickened cell wall compared to infection hyphae. We demonstrate that take-all control is achieved via local but not systemic host changes in response to prior G. hyphopodioides root colonisation. A time-course wheat RNA sequencing analysis revealed extensive transcriptional reprogramming in G. hyphopodioides colonised tissues, characterised by a striking downregulation of key cell-wall related genes, including cellulose synthase (CESA), and xyloglucan endotransglucosylase/hydrolase (XTH) genes. In the absence of take-all resistant wheat cultivars or non-virulent G. tritici strains, studying closely related non-pathogenic G. hyphopodioides provides a much-needed avenue to elucidate take-all resistance mechanisms in wheat |
Keywords | Fungal biocontrol; Cell wall modifications; Wheat defences; Root pathogen; Root endophyte; Wheat root transcriptomes |
Year of Publication | 2024 |
Journal | Frontiers in Plant Science |
bioRxiv | |
Journal citation | 15 |
Digital Object Identifier (DOI) | https://doi.org/10.3389/fpls.2024.1444271 |
https://doi.org/10.1101/2023.11.23.568424 | |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Department of Environment, Food and Rural Affairs | |
Funder project or code | Nottingham-Rothamsted Doctoral Training Partnership |
DFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience | |
BB/CCG2280/1 | |
Delivering Sustainable Wheat | |
Delivering Sustainable Wheat (WP2): Delivering Resilience to Biotic Stress | |
Semiochemical-based alternative concepts for the management of wireworms | |
Accepted author manuscript | |
Output status | Published |
Publication dates | |
Online | 27 May 2024 |
Publication process dates | |
Accepted | 06 Aug 2024 |
Publisher | Cold Spring Harbor Lab Press, Publications Dept |
Frontiers Media SA | |
ISSN | 1664-462X |
Permalink - https://repository.rothamsted.ac.uk/item/9906q/a-fungal-endophyte-induces-local-cell-wall-mediated-resistance-in-wheat-roots-against-take-all-disease