Virus-induced gene silencing in wheat and related monocot species

B - Book chapters etc edited externally

Panwar, V. and Kanyuka, K. 2022. Virus-induced gene silencing in wheat and related monocot species. in: Mysore, K. S. and Senthil-Kumar, M. (ed.) Plant Gene Silencing Humana Press Inc.

AuthorsPanwar, V. and Kanyuka, K.
EditorsMysore, K. S. and Senthil-Kumar, M.

Advances made in genome sequencing projects and structural genomics are generating large repertoire of candidate genes in plants associated with specific agronomic traits. Rapid and high-throughput functional genomics approaches are therefore needed to validate the biological function of these genes especially for agronomically important crops beyond the few model plant species. This can be achieved by utilising available gene knock-out or transgenic methodologies, but these can take considerable time and effort particularly in crops with large and complex genomes such as wheat. Therefore, any tool that expedites the validation of gene function is of particular benefit especially in cereal crop plants that are genetically difficult to transform. One such reverse genetics tool is virus-induced gene silencing (VIGS) which relies on the plants’ natural antiviral RNA silencing defence mechanism. VIGS is used to down regulate target gene expression in a transient manner which persists long enough to determine its effect on a specific trait. VIGS based on Barley stripe mosaic virus (BSMV) is rapid, powerful, efficient, and relatively inexpensive tool for the analysis of gene function in cereal species. Here we present detailed protocols for BSMV-mediated VIGS for robust gene silencing in bread wheat and related species.

KeywordsVIGS; Gene silencing; BSMV; Cereals; Wheat; Functional genomics
Year of Publication2022
Book titlePlant Gene Silencing
PublisherHumana Press Inc
SeriesMethods in Molecular Biology 2408
Digital Object Identifier (DOI)
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online25 Mar 2022

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