Isolation and identification of floral attractants from a nectar plant for the dried bean beetle, Acanthoscelides obtectus (Coleoptera: Chrysomelidae, Bruchinae)

A - Papers appearing in refereed journals

Vuts, J., Woodcock, C. M., Caulfield, J. C., Powers, S. J., Pickett, J. A. and Birkett, M. A. 2018. Isolation and identification of floral attractants from a nectar plant for the dried bean beetle, Acanthoscelides obtectus (Coleoptera: Chrysomelidae, Bruchinae). Pest Management Science.

AuthorsVuts, J., Woodcock, C. M., Caulfield, J. C., Powers, S. J., Pickett, J. A. and Birkett, M. A.
Abstract

BACKGROUND:The response of virgin females of the legume pest Acanthoscelides obtectus (Coleoptera: Bruchidae) to headspace extracts of volatiles collected from flowers of a nectar plant, Daucus carota, was investigated using behaviour (four-arm olfactometry) and coupled gas chromatography–electroantennography (GC-EAG).
RESULTS: Odours from inflorescences were significantly more attractive to virgin female beetles than clean air. Similarly, a sample of volatile organic compounds (VOCs) collected by air entrainment (dynamic headspace collection) was more attractive to beetles than a solvent control. In coupled GC-EAG experiments with beetle antennae and the VOC extract, six components howed AG activity. Using coupled GC–mass spectrometry (GC–MS) and GC peak enhancement with authentic standards, the components were identified as 𝜶-pinene (S:R 16:1), sabinene, myrcene, limonene (S:R 1:3), terpinolene and (S)-bornyl acetate. Females preferred the synthetic blend of D. carota EAG-active volatiles to the solvent control in bioassays. When compared directly, odours of D. carota inflorescences elicited stronger positive behaviour than the synthetic blend.
CONCLUSION: This is the first report of behaviourally active volatiles linked to pollen location for A. obtectus, and development of the six-component blend is being pursued, which could underpin the design of semiochemical-based field management approaches against this major pest of stored products.

KeywordsAcanthoscelides obtectus ; Plant volatiles ; Gas chromatography–electroantennography (GC-EAG); Attractant; Chemical ecology; Behavioural assays
Year of Publication2018
JournalPest Management Science
Digital Object Identifier (DOI)doi:10.1002/ps.4903
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeBBSRC Strategic Programme in Smart Crop Protection
Delivering Sustainable Systems (SS) [ISPG]
Output statusE-publication ahead of print
Publication process dates
Accepted08 Mar 2018
PublisherWiley-Blackwell
Wiley
Copyright licenseCC BY
ISSN1526-498X

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