Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean

A - Papers appearing in refereed journals

Parmezan, T. R., Brito Junior, S. L., De Carvalho, K., De Aquino, M., Birkett, M. A., Pickett, J. A., De Oliveira Nunes, E., Abdelnor, R. V., Hoffmann Campo, C. B. and Marcelino-Guimaraes, F. C. 2020. Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean. Genetics and Molecular Biology. 43 (4), p. e20190388. https://doi.org/10.1590/1678-4685-gmb-2019-0388

AuthorsParmezan, T. R., Brito Junior, S. L., De Carvalho, K., De Aquino, M., Birkett, M. A., Pickett, J. A., De Oliveira Nunes, E., Abdelnor, R. V., Hoffmann Campo, C. B. and Marcelino-Guimaraes, F. C.
Abstract

Terpenes produced by plants comprise a diverse range of secondary metabolites, including volatile organic compounds (VOCs). Terpene VOC production may be altered after damage or by biological stimuli such as bacterial, fungal and insects, and subsequent triggering of plant defense responses. These VOCs originate in plants from two independent pathways: the mevalonate and the methylerythritol phosphate pathways, which utilize dimethylallyl and isopentenyl diphosphates to form the terpenoidal precursors. Phakopsora pachyrhizi fungi causes Asian soybean rust, limiting soybean production and resulting in losses of up to 80% if no control strategies are applied. By using a transcriptome datasets, we investigated the regulation of genes of the mevalonate pathway under different biotic stresses. We studied the impact of P. pachyrhizi infection in vivo expression profile of genes involved in terpenoid and glyceollin biosynthesis in genotypes harboring different resistance genes (Rpp), and across the infection cycle. In addition, we used UPLC and
UPGC analysis to evaluate glyceollin and VOC production, respectively, to identify metabolites associated with soybean responses to pathogen infection. The regulation of soybean genes involved in terpene production was influenced by genotypes, depending on the Rpp gene, while glyceollin was induced in all genotypes. Furthermore, a sesquiterpene
was identified as a potential marker associated with rust symptoms on soybean.

KeywordsRust; Glycine max; Terpenes; Glyceollin
Year of Publication2020
JournalGenetics and Molecular Biology
Journal citation43 (4), p. e20190388
Digital Object Identifier (DOI)https://doi.org/10.1590/1678-4685-gmb-2019-0388
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeUK - Brazil partnership on the chemical ecology of crop-plant/rust-pathogen interactions for underpinning novel crop protection strategies
Publisher's version
Output statusPublished
Publication dates
Online06 Nov 2020
Publication process dates
Accepted08 Jul 2020
PublisherBrazilian Society of Genetics
ISSN0100-8455

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