The role of ZIP transporters and group F bZIP transcription factors in the Zn-deficiency response of wheat (Triticum aestivum).

A - Papers appearing in refereed journals

Evens, N. P., Buchner, P. H., Williams, L. E. and Hawkesford, M. J. 2017. The role of ZIP transporters and group F bZIP transcription factors in the Zn-deficiency response of wheat (Triticum aestivum). The Plant Journal. 92 (2), pp. 291-304. https://doi.org/10.1111/tpj.13655

AuthorsEvens, N. P., Buchner, P. H., Williams, L. E. and Hawkesford, M. J.
Abstract

Understanding the molecular basis of zinc (Zn) uptake and transport in staple cereal crops is critical for improving both Zn content and tolerance to low-Zn soils. This study demonstrates the importance of group F bZIP transcription factors and ZIP transporters in responses to Zn deficiency in wheat (Triticum aestivum). Seven group F TabZIP genes and 14 ZIPs with homeologs were identified in hexaploid wheat. Promoter analysis revealed the presence of Zn-deficiency-response elements (ZDREs) in a number of the ZIPs. Functional complementation of the zrt1/zrt2 yeast mutant by TaZIP3, -6, -7, -9 and -13 supported an ability to transport Zn. Group F TabZIPs contain the group-defining cysteine–histidine-rich motifs, which are the predicted binding site of Zn2+ in the Zn-deficiency response. Conservation of these motifs varied between the TabZIPs suggesting that individual TabZIPs may have specific roles in the wheat Zn-homeostatic network. Increased expression in response to low Zn levels was observed for several of the wheat ZIPs and bZIPs; this varied temporally and spatially suggesting specific functions in the response mechanism. The ability of the group F TabZIPs to bind to specific ZDREs in the promoters of TaZIPs indicates a conserved mechanism in monocots and dicots in responding to Zn deficiency. In support of this, TabZIPF1-7DL and TabZIPF4-7AL afforded a strong level of rescue to the Arabidopsis hypersensitive bzip19 bzip23 double mutant under Zn deficiency. These results provide a greater understanding of Zn-homeostatic mechanisms in wheat, demonstrating an expanded repertoire of group F bZIP transcription factors, adding to the complexity of Zn homeostasis.

KeywordsZinc; Micronutrient; Biofortification; ZIP transporter; Membrane transport; bZIP; Wheat (Triticum aestivum); Transcription factor
Year of Publication2017
JournalThe Plant Journal
Journal citation92 (2), pp. 291-304
Digital Object Identifier (DOI)https://doi.org/10.1111/tpj.13655
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or code[20:20 Wheat] Maximising yield potential of wheat
Designing Future Wheat (DFW) [ISPG]
NUE/phenotyping (WP1) [DFW - ISPG]
BB/J0414451/1
DFW - Designing Future Wheat - Work package 1 (WP1) - Increased efficiency and sustainability
Accepted author manuscript
Output statusPublished
Publication dates
Online03 Aug 2017
Publication process dates
Accepted28 Jul 2017
PublisherJohn Wiley & Sons Inc
Wiley-Blackwell
Copyright licenseCC BY
ISSN0960-7412

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