The role of ZIP transporters and group F bZIP transcription factors in the Zn-deficiency response of wheat (Triticum aestivum).

A - Papers appearing in refereed journals

Evens, N. P., Buchner, P. H., Williams, L. E. and Hawkesford, M. J. 2017. The role of ZIP transporters and group F bZIP transcription factors in the Zn-deficiency response of wheat (Triticum aestivum). The Plant Journal. 92 (2), pp. 291-304.

AuthorsEvens, N. P., Buchner, P. H., Williams, L. E. and Hawkesford, M. J.
Abstract

Understanding the molecular basis of zinc (Zn) uptake and transport in staple cereal crops is critical for improving both Zn content and tolerance to low-Zn soils. This study demonstrates the importance of group F bZIP transcription factors and ZIP transporters in responses to Zn deficiency in wheat (Triticum aestivum). Seven group F TabZIP genes and 14 ZIPs with homeologs were identified in hexaploid wheat. Promoter analysis revealed the presence of Zn-deficiency-response elements (ZDREs) in a number of the ZIPs. Functional complementation of the zrt1/zrt2 yeast mutant by TaZIP3, -6, -7, -9 and -13 supported an ability to transport Zn. Group F TabZIPs contain the group-defining cysteine–histidine-rich motifs, which are the predicted binding site of Zn2+ in the Zn-deficiency response. Conservation of these motifs varied between the TabZIPs suggesting that individual TabZIPs may have specific roles in the wheat Zn-homeostatic network. Increased expression in response to low Zn levels was observed for several of the wheat ZIPs and bZIPs; this varied temporally and spatially suggesting specific functions in the response mechanism. The ability of the group F TabZIPs to bind to specific ZDREs in the promoters of TaZIPs indicates a conserved mechanism in monocots and dicots in responding to Zn deficiency. In support of this, TabZIPF1-7DL and TabZIPF4-7AL afforded a strong level of rescue to the Arabidopsis hypersensitive bzip19 bzip23 double mutant under Zn deficiency. These results provide a greater understanding of Zn-homeostatic mechanisms in wheat, demonstrating an expanded repertoire of group F bZIP transcription factors, adding to the complexity of Zn homeostasis.

KeywordsZinc; Micronutrient; Biofortification; ZIP transporter; Membrane transport; bZIP; Wheat (Triticum aestivum); Transcription factor
Year of Publication2017
JournalThe Plant Journal
Journal citation92 (2), pp. 291-304
Digital Object Identifier (DOI)doi:10.1111/tpj.13655
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or code[20:20 Wheat] Maximising yield potential of wheat
Designing Future Wheat (DFW) [ISPG]
NUE/phenotyping (WP1) [DFW - ISPG]
BB/J0414451/1
DFW - Designing Future Wheat - Work package 1 (WP1) - Increased efficiency and sustainability
Accepted author manuscript
Output statusPublished
Publication dates
Online03 Aug 2017
Publication process dates
Accepted28 Jul 2017
PublisherJohn Wiley & Sons Inc
Wiley-Blackwell
Copyright licenseCC BY
ISSN0960-7412

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