System analysis of metabolism and the transcriptome in Arabidopsis thaliana roots reveals differential co-regulation upon iron, sulfur and potassium deficiency

A - Papers appearing in refereed journals

Forieri, I., Sticht, C., Reichelt, M., Gretz, N., Hawkesford, M. J., Malagoli, M., Wirtz, M. and Hell, R. 2017. System analysis of metabolism and the transcriptome in Arabidopsis thaliana roots reveals differential co-regulation upon iron, sulfur and potassium deficiency. Plant, Cell & Environment. 40 (1), pp. 95-107.

AuthorsForieri, I., Sticht, C., Reichelt, M., Gretz, N., Hawkesford, M. J., Malagoli, M., Wirtz, M. and Hell, R.
Abstract

Deprivation of mineral nutrients causes significant retardation of plant growth. This retardation is associated with nutrient-specific and general stress-induced transcriptional responses. In this study, we adjusted the external supply of iron, potassium and sulfur to cause the same retardation of shoot growth. Nevertheless, limitation by individual nutrients resulted in specific morphological adaptations and distinct shifts within the root metabolite fingerprint. The metabolic shifts affected key metabolites of primary metabolism and the stress-related phytohormones, jasmonic, salicylic and abscisic acid. These phytohormone signatures contributed to specific nutrient deficiency-induced transcriptional regulation. Limitation by the micronutrient iron caused the strongest regulation and affected 18% of the root transcriptome. Only 130 genes were regulated by all nutrients. Specific co-regulation between the iron and sulfur metabolic routes upon iron or sulfur deficiency was observed. Interestingly, iron deficiency caused regulation of a different set of genes of the sulfur assimilation pathway compared with sulfur deficiency itself, which demonstrates the presence of specific signal-transduction systems for the cross-regulation of the pathways. Combined iron and sulfur starvation experiments demonstrated that a requirement for a specific nutrient can overrule this cross-regulation. The comparative metabolomics and transcriptomics approach used dissected general stress from nutrient-specific regulation in roots of Arabidopsis.

KeywordsPlant Sciences
Year of Publication2017
JournalPlant, Cell & Environment
Journal citation40 (1), pp. 95-107
Digital Object Identifier (DOI)doi:10.1111/pce.12842
PubMed ID27726154
Open accessPublished as non-open access
FunderDFG
Funder project or codeWheat
Publication dates
Online10 Oct 2016
PublisherWiley
Wiley-Blackwell
ISSN0140-7791

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