A - Papers appearing in refereed journals
Zhao, D., Derkx, A. P., Liu, D-C., Buchner, P. H. and Hawkesford, M. J. 2015. Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat. Plant Biology. 17 (4), pp. 904-913.
|Authors||Zhao, D., Derkx, A. P., Liu, D-C., Buchner, P. H. and Hawkesford, M. J.|
Increasing the duration of leaf photosynthesis during grain filling using slow-senescing functional stay-green phenotypes is a possible route for increasing grain yields in wheat (Triticum aestivum L.). However, delayed senescence may negatively affect nutrient remobilisation and hence reduce grain protein concentrations and grain quality. A novel NAC1-type transcription factor (hereafter TaNAC-S) was identified in wheat, with gene expression located primarily in leaf/sheath tissues, which decreased during post-anthesis leaf senescence. Expression of TaNAC-S in the second leaf correlated with delayed senescence in two doubled-haploid lines of an AvalonxCadenza population (lines 112 and 181), which were distinct for leaf senescence. Transgenic wheat plants overexpressing TaNAC-S resulted in delayed leaf senescence (stay-green phenotype). Grain yield, aboveground biomass, harvest index and total grain N content were unaffected, but NAC over-expressing lines had higher grain N concentrations at similar grain yields compared to non-transgenic controls. These results indicate that TaNAC-S is a negative regulator of leaf senescence, and that delayed leaf senescence may lead not only to increased grain yields but also to increased grain protein concentrations.
|Year of Publication||2015|
|Journal citation||17 (4), pp. 904-913|
|Digital Object Identifier (DOI)||doi:10.1111/plb.12296|
|Open access||Published as ‘gold’ (paid) open access|
|Funder||Lawes Agricultural Trust (LAT)|
|Chinese Scholarship Council|
|Biotechnology and Biological Sciences Research Council|
|Funder project or code||Wheat|
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