Root architecture and leaf photosynthesis traits and associations with nitrogen-use efficiency in landrace-derived lines in wheat

A - Papers appearing in refereed journals

Kareem, S. H. S., Hawkesford, M. J., DeSilva, J., Weerasinghe, M., Wells, D. M., Pound, M. P., Atkinson, J. A. and Foulkes, M. J. 2022. Root architecture and leaf photosynthesis traits and associations with nitrogen-use efficiency in landrace-derived lines in wheat. European Journal of Agronomy. 140, p. 126603. https://doi.org/10.1016/j.eja.2022.126603

AuthorsKareem, S. H. S., Hawkesford, M. J., DeSilva, J., Weerasinghe, M., Wells, D. M., Pound, M. P., Atkinson, J. A. and Foulkes, M. J.
Abstract

Root system architecture (RSA) is important in optimizing the use of nitrogen. High-throughput phenotyping techniques may be used to study root system architecture traits under controlled environments. A root phenotyping platform, consisting of germination paper-based pouch and wick coupled with image analysis, was used to characterize root seedling traits in 30 landrace-derived bread wheat genotypes and the bread wheat parent Paragon under hydroponic high N (HN) and low N (LN) conditions. In addition, two glasshouse experiments under HN and LN conditions were carried out to measure whole plant performance including flag-leaf photosynthetic rate, N uptake and biomass per plant for 13 wheat genotypes of which eight were common with those in the root phenotyping hydroponic experiment. There were significant differences in RSA traits between genotypes for seminal root number per plant, lateral root number per plant, seminal root length per plant and seminal root angle, with transgressive segregation for landrace-derived lines above the elite parental cultivar Paragon under HN and LN conditions. In the glasshouse experiments, genetic variation in flag-leaf photosynthesis rate was found in landrace-derived genotypes in the range 25.9–33.3 µmol m−2 s−1 under HN and in N uptake in the ranges 0.37–0.48 g N plant−1and 0.21–0.30 g plant−1 under HN and LN conditions, respectively (P < 0.05), with transgressive segregation above Paragon. Plant Nitrogen Nutrition Index also showed transgressive segregation in the landrace-derived lines above Paragon under HN and LN conditions. Greater maximum root depth and more lateral roots per plant in the hydroponic screen were each correlated with increased biomass per plant under LN conditions. Results from this study demonstrated genetic variation for seedling RSA traits in landrace-derived lines above the elite parental cultivar Paragon, which potentially could be utilized to improve N-use efficiency in breeding programmes.

KeywordsRoot system architecture; Leaf photosynthesis; Wheat; Landraces; Yield; N-use efficiency
Year of Publication2022
JournalEuropean Journal of Agronomy
Journal citation140, p. 126603
Digital Object Identifier (DOI)https://doi.org/10.1016/j.eja.2022.126603
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeIndo-UK Centre for the improvement of Nitrogen use Efficiency in Wheat (INEW)
Output statusPublished
Publication dates
Online26 Aug 2022
Publication process dates
Accepted05 Aug 2022
PublisherElsevier Science Bv
ISSN1161-0301

Permalink - https://repository.rothamsted.ac.uk/item/98q83/root-architecture-and-leaf-photosynthesis-traits-and-associations-with-nitrogen-use-efficiency-in-landrace-derived-lines-in-wheat

Restricted files

Publisher's version

Under embargo indefinitely

26 total views
0 total downloads
6 views this month
0 downloads this month