Opposite variations in fumarate and malate dominate metabolic phenotypes of Arabidopsis salicylate mutants with abnormal biomass under chilling

A - Papers appearing in refereed journals

Scott, I. M., Ward, J. L., Miller, S. J. and Beale, M. H. 2014. Opposite variations in fumarate and malate dominate metabolic phenotypes of Arabidopsis salicylate mutants with abnormal biomass under chilling. Physiologia Plantarum. 152 (4), pp. 660-674.

AuthorsScott, I. M., Ward, J. L., Miller, S. J. and Beale, M. H.
Abstract

In chilling conditions (5 degrees C), salicylic acid (SA)-deficient mutants (sid2, eds5 and NahG) of Arabidopsis thaliana produced more biomass than wild type (Col-0), whereas the SA overproducer cpr1 was extremely stunted. The hypothesis that these phenotypes were reflected in metabolism was explored using 600MHz H-1 nuclear magnetic resonance (NMR) analysis of unfractionated polar shoot extracts. Biomass-related metabolic phenotypes were identified as multivariate data models of these NMR fingerprints'. These included principal components that correlated with biomass. Also, partial least squares-regression models were found to predict the relative size of plants in previously unseen experiments in different light intensities, or relative size of one genotype from the others. The dominant signal in these models was fumarate, which was high in SA-deficient mutants, intermediate in Col-0 and low in cpr1 at 5 degrees C. Among signals negatively correlated with biomass, malate was prominent. Abundance of transcripts of the FUM2 cytosolic fumarase (At5g50950) showed strong positive correlation with fumarate levels and with biomass, whereas no significant differences were found for the FUM1 mitochondrial fumarase (At2g47510). It was confirmed that the morphological effects of SA under chilling find expression in the metabolome, with a role of fumarate highlighted.

KeywordsPlant Sciences
Year of Publication2014
JournalPhysiologia Plantarum
Journal citation152 (4), pp. 660-674
Digital Object Identifier (DOI)doi:10.1111/ppl.12210
PubMed ID24735077
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeMETRO
Cropping Carbon (CC) [ISPG]
PublisherWiley
ISSN0031-9317

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