Opposite fates of the purine metabolite allantoin under water and nitrogen limitations in bread wheat

A - Papers appearing in refereed journals

Casartelli, A., Melino, V.J., Baumann, U., Riboni, M., Suchecki, R., Jayasinghe, N.S., Mendis, H., Watanabe, M., Erban, A., Zuther, E., Hoefgen, R., Roessner, U., Okamoto, M. and Heuer, S. 2019. Opposite fates of the purine metabolite allantoin under water and nitrogen limitations in bread wheat. Plant Molecular Biology. 99 (4-5), pp. 477-497. https://doi.org/10.1007/s11103-019-00831-z

AuthorsCasartelli, A., Melino, V.J., Baumann, U., Riboni, M., Suchecki, R., Jayasinghe, N.S., Mendis, H., Watanabe, M., Erban, A., Zuther, E., Hoefgen, R., Roessner, U., Okamoto, M. and Heuer, S.
Abstract

Key message:
Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen
supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress.
Abstract:
The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleotides) and is known for its housekeeping role in nitrogen (N) recycling and also for its function in N transport and storage in nodulated legumes. Allantoin was also shown to differentially accumulate upon abiotic stress in a range of plant species but little is known about its role in cereals. To address this, purine catabolic pathway genes were identified in hexaploid bread wheat and their chromosomal location was experimentally validated. A comparative study of two Australian bread wheat genotypes revealed a highly significant increase of allantoin (up to 29-fold) under drought. In contrast, allantoin significantly decreased (up to 22-fold) in response to N deficiency. The observed changes were accompanied by transcriptional adjustment of key purine catabolic genes, suggesting that the recycling of purine-derived N is tightly regulated under stress. We propose opposite fates of allantoin in plants under stress: the accumulation of allantoin under drought circumvents its degradation to ammonium (NH4+) thereby preventing N losses. On the other hand, under N deficiency, increasing the NH4+ liberated via allantoin catabolism contributes towards the maintenance of N homeostasis.

KeywordsAllantoin; Drought ; Nitrogen deficiency; Nutrient recycling; Purine catabolism; Triticum aestivum
Year of Publication2019
JournalPlant Molecular Biology
Journal citation99 (4-5), pp. 477-497
Digital Object Identifier (DOI)https://doi.org/10.1007/s11103-019-00831-z
PubMed ID30721380
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 1 (WP1) - Increased efficiency and sustainability
Publisher's version
Accepted author manuscript
Output statusPublished
Publication dates
Online05 Feb 2019
Publication process dates
Accepted24 Jan 2019
PublisherSpringer
Copyright licenseCC BY
ISSN0167-4412

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