What makes a weed a weed? How virus-mediated reverse genetics can help to explore the genetics of weediness

A - Papers appearing in refereed journals

Macgregor, D. 2020. What makes a weed a weed? How virus-mediated reverse genetics can help to explore the genetics of weediness. Outlooks on Pest Management. 31 (5), pp. 223 - 229. https://doi.org/DOI:10.1564/v31_oct_07

AuthorsMacgregor, D.
Abstract

Reverse genetics investigates what a gene does by testing how the plant responds when the specific gene is changed. These techniques have been in use for decades to assess whether a given gene underpins interesting phenotypes and gain insight into the function of gene networks and families. Weed science has only recently entered the “genomic era” in which genomic and reverse genetics approaches are used to address hypotheses. This review focuses on two reverse genetic techniques used on a variety of plants including agricultural weeds, virus-induced gene silencing (VIGS) and virus-mediated overexpression (VOX), explaining the biology behind them and highlighting how these tools may be used for gene function validation in weed species for which no other transgenic approaches have been developed.

KeywordsHerbicide Resistance; Reverse Genetics; Transient Transformation; Virus-Mediated Reverse Genetics (VMRG); Virus-Induced Gene Silencing (VIGS); Virus Mediated Overexpression (VOX); Weed Molecular Biology
Year of Publication2020
JournalOutlooks on Pest Management
Journal citation31 (5), pp. 223 - 229
Digital Object Identifier (DOI)https://doi.org/DOI:10.1564/v31_oct_07
Open accessPublished as bronze (free) open access
FunderBBSRC Industrial Strategy Challenge
Funder project or codeBBSRC Strategic Programme in Smart Crop Protection
Publisher's version
Output statusPublished
Publication dates
Online13 Oct 2020
Publication process dates
Accepted13 Oct 2020
PublisherResearch Information
ISSN1743-1034

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