ICE1 and ZOU determine the depth of primary seed dormancy in Arabidopsis independently of their role in endosperm development

A - Papers appearing in refereed journals

MacGregor, D. R., Zhang, N., Iwasaki, M., Chen, M., Dave, A., Lopez-Molina, L. and Penfield, S. 2018. ICE1 and ZOU determine the depth of primary seed dormancy in Arabidopsis independently of their role in endosperm development. The Plant Journal. 98 (2), pp. 277-290. https://doi.org/10.1111/tpj.14211

AuthorsMacGregor, D. R., Zhang, N., Iwasaki, M., Chen, M., Dave, A., Lopez-Molina, L. and Penfield, S.
Abstract

Seed dormancy is a widespread and key adaptive trait that is essential for the establishment of soil seed banks and prevention of preharvest sprouting. Herein we demonstrate that the endosperm-expressed transcription factors ZHOUPI (ZOU) and INDUCER OF CBF EXPRESSION1 (ICE1) play a role in determining primary dormancy depth in Arabidopsis. We show that ice1 or zou increases seed dormancy and the double mutant has an additive phenotype. The increased dormancy is associated with increased ABA levels, and can be separated genetically from their role in endosperm maturation, because loss of ABA biosynthesis or DELAY OF GERMINATION 1 reverses the dormancy phenotype without affecting the aberrant seed morphology. Consistent with these results, ice1 endosperms had an increased capacity for preventing embryo greening, a phenotype previously associated with an increase in endospermic ABA levels. Although ice1 changes the expression of many genes including some in ABA biosynthesis, catabolism and/or signalling, only ABA INSENSITIVE 3 is significantly misregulated in ice1 mutants. We also demonstrate that ICE1 binds to and inhibits expression of the ABA INSENSITIVE 3. Our data demonstrate that Arabidopsis ICE1 and ZOU determine the depth of primary dormancy during maturation independently of their effect on endosperm development.

KeywordsABA ; AB13; Endosperm consumption; ICE1; Primary dormancy; Seed development; ZOU
Year of Publication2018
JournalThe Plant Journal
Journal citation98 (2), pp. 277-290
Digital Object Identifier (DOI)https://doi.org/10.1111/tpj.14211
PubMed ID30570804
Open accessPublished as ‘gold’ (paid) open access
FunderSwiss National Science Foundation
Biotechnology and Biological Sciences Research Council
Funder project or codeBBSRC Strategic Programme in Smart Crop Protection
Controlling seed coat plasticity for seed quality in industry
Publisher's version
Accepted author manuscript
Output statusPublished
Publication dates
Online20 Dec 2018
Publication process dates
Accepted12 Dec 2018
Copyright licenseCC BY
PublisherWiley-Blackwell
ISSN0960-7412

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