CTAB DNA Extraction and Genotyping-by-Sequencing to Map Meiotic Crossovers in Plants

B - Book chapters etc edited externally

Lambing, C. 2022. CTAB DNA Extraction and Genotyping-by-Sequencing to Map Meiotic Crossovers in Plants. in: Lambing, C. (ed.) Plant Gametogenesis New York Springer Nature. pp. 43-53

AuthorsLambing, C.
EditorsLambing, C.

Reciprocal DNA crossovers between chromosomes form new allelic combinations and contribute to the formation of novel genetic diversity. Crossovers are formed during meiosis of germ cells and these recombination events have influenced plant genome evolution and are used during breeding to create improved plant varieties. Meiotic crossovers are not uniformly formed across the genome but instead occur in regions with low nucleosome density. The recombination landscape differs between the model plant organism Arabidopsis thaliana and crops such as rice and maize. Genotyping-by-sequencing is a technique that can detect crossover location and provide information on the recombination landscape genome-wide. This technique can be used to compare crossover position between ecotypes, species, and mutant lines to gain information on factors controlling meiotic recombination. In this protocol, we describe the steps to purify DNA from plant tissue, prepare 96 DNA libraries in parallel and perform quality control before next-generation sequencing.

KeywordsCTAB DNA extraction; Crossover; DNA sequencing; Genotyping-by-sequencing; Meiosis; Plant; Recombination
Page range43-53
Year of Publication2022
Book titlePlant Gametogenesis
PublisherSpringer Nature
Humana Press Inc
Place of publicationNew York
SeriesMethods and Protocols vol 2484
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-0716-2253-7_4
PubMed ID35461443
Web address (URL)https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_4
FunderBiotechnology and Biological Sciences Research Council
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online24 Apr 2022

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