Incorrect recombination partner associations contribute to meiotic instability of neo-allopolyploid Arabidopsis suecica

A - Papers appearing in refereed journals

Cheron, F., Petiot, V., Lambing, C., White, C. and Serra, H. 2023. Incorrect recombination partner associations contribute to meiotic instability of neo-allopolyploid Arabidopsis suecica. New Phytologist. https://doi.org/10.1111/nph.19487

AuthorsCheron, F., Petiot, V., Lambing, C., White, C. and Serra, H.
Abstract

Combining two or more related homoeologous genomes in a single nucleus, newly formed allopolyploids must rapidly adapt meiosis to restore balanced chromosome segregation, production of euploid gametes and fertility. The poor fertility of such neo-allopolyploids thus strongly selects for the limitation or avoidance of genetic crossover formation between homoeologous chromosomes. In this study, we have reproduced the interspecific hybridization between Arabidopsis thaliana and Arabidopsis arenosa leading to the allotetraploid Arabidopsis suecica and have characterized the first allopolyploid meioses. First-generation neo-allopolyploid siblings vary considerably in fertility, meiotic behavior and levels of homoeologous recombination. We show that centromere dynamics at early meiosis is altered in synthetic neo-allopolyploids compared with evolved A. suecica, with a significant increase in homoeologous centromere interactions at zygotene. At metaphase I, the presence of multivalents involving homoeologous chromosomes confirms that homoeologous recombination occurs in the first-generation synthetic allopolyploid plants and this is associated with a significant reduction in homologous recombination, compared to evolved A. suecica. Together, these data strongly suggest that the fidelity of recombination partner choice, likely during the DNA invasion step, is strongly impaired during the first meiosis of neo-allopolyploids and requires subsequent adaptation.

KeywordsArabidopsis suecica; Allopolyploid; Crossover; Homoeologous chromosomes; Meiosis; Pairing; Polyploidy; Recombination
Year of Publication2023
JournalNew Phytologist
Digital Object Identifier (DOI)https://doi.org/10.1111/nph.19487
PubMed ID38158491
Web address (URL)https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19487
Open accessPublished as ‘gold’ (paid) open access
Publisher's version
Output statusPublished
Publication dates
Online29 Dec 2023
Publication process dates
Accepted29 Nov 2023
PublisherWiley
ISSN0028-646X

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