MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters

Terlouw, B. R., Blin, K., Navarro-Munoz, J. C., Avalon, N. E., Chevrette, M. G., Egbert, S., Meijer, D., Recchia, M. J. J., Reitz, Z. L., Santen, J. A. V., Selem-Mojica, N., Torring, T., Zaroubi, L., Alanjary, M., Aleti, G., Aguilar, C., Al-Salihi, S. A. A., Augustijn, H. E., Avelar-Rivas, J. A., Avitia-Domınguez, L. A., Barona-Gomez, F., Bernaldo-Aguero, J., Bielinski, V. A., Biermann, F., Booth, T. J., Bravo, V. J. C., Castelo-Branco, R., Chagas, F. O., Cruz-Morales, P., Du, C., Duncan, K. R., Gavriilidou, A., Gayrard, D., Gutierrez-Garcıa, K., Haslinger, K., Helfrich, E. J. N., Hooft, J. J. V. D., Jati, A. P., Kalkreuter, E., Kalyvas, N., Kang, K. B., Kautsar, S., Kim, W., Kunjapur, A. M., Lee, W-S., Li, Y., Lin, G., Loureiro, C., Louwen, J. J. R., Louwen, N. L., Lund, G., Parra, J., Philmus, B., Pourmohsenin, B., Pronk, L. J. U., Rego, A., Rex, D. A. B., Robinson, S., Rosas-Becerra, L. R., Roxborough, E. T., Schorn, M. A., Scobie, D. J., Sing, K. S., Sokolova, N., Tang, X., Udwary, D., Vigneshwari, A., Vind, K., Vromans, S. P. J. M., Waschulin, V., Williams, S. E., Winter, J. M., Witte, T. E., Yang, H. X. D., Yu, J., Zdouc, M., Zhong, Z., Collemare, J., Linington, R. G., Weber, T. and Medema, M. H. 2023. MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters. Nucleic Acids Research. 51 (D1), pp. D606-D610. https://doi.org/10.1093/nar/gkac1049

Authors	Terlouw, B. R., Blin, K., Navarro-Munoz, J. C., Avalon, N. E., Chevrette, M. G., Egbert, S., Meijer, D., Recchia, M. J. J., Reitz, Z. L., Santen, J. A. V., Selem-Mojica, N., Torring, T., Zaroubi, L., Alanjary, M., Aleti, G., Aguilar, C., Al-Salihi, S. A. A., Augustijn, H. E., Avelar-Rivas, J. A., Avitia-Domınguez, L. A., Barona-Gomez, F., Bernaldo-Aguero, J., Bielinski, V. A., Biermann, F., Booth, T. J., Bravo, V. J. C., Castelo-Branco, R., Chagas, F. O., Cruz-Morales, P., Du, C., Duncan, K. R., Gavriilidou, A., Gayrard, D., Gutierrez-Garcıa, K., Haslinger, K., Helfrich, E. J. N., Hooft, J. J. V. D., Jati, A. P., Kalkreuter, E., Kalyvas, N., Kang, K. B., Kautsar, S., Kim, W., Kunjapur, A. M., Lee, W-S., Li, Y., Lin, G., Loureiro, C., Louwen, J. J. R., Louwen, N. L., Lund, G., Parra, J., Philmus, B., Pourmohsenin, B., Pronk, L. J. U., Rego, A., Rex, D. A. B., Robinson, S., Rosas-Becerra, L. R., Roxborough, E. T., Schorn, M. A., Scobie, D. J., Sing, K. S., Sokolova, N., Tang, X., Udwary, D., Vigneshwari, A., Vind, K., Vromans, S. P. J. M., Waschulin, V., Williams, S. E., Winter, J. M., Witte, T. E., Yang, H. X. D., Yu, J., Zdouc, M., Zhong, Z., Collemare, J., Linington, R. G., Weber, T. and Medema, M. H.
Abstract	With an ever-increasing amount of (meta)genomic data being deposited in sequence databases,(meta)genome mining for natural product biosynthetic pathways occupies a critical role in the discovery of novel pharmaceutical drugs, crop protection agents and biomaterials. The genes that encode these pathways are often organised into biosynthetic gene clusters (BGCs). In 2015, we defined the Minimum Information about a Biosynthetic Gene cluster (MIBiG): a standardised data format that describes the minimally required information to uniquely characterise a BGC. We simultaneously constructed an accompanying online database of BGCs, which has since been widely used by the community as a reference dataset for BGCs and was expanded to 2021 entries in 2019 (MIBiG 2.0). Here, we describe MIBiG 3.0, a database update comprising large-scale validation and re-annotation of existing entries and 661 new entries. Particular attention was paid to the annotation of compound structures and biological activities, as well as protein domain selectivities. Together, these new features keep the database upto-date, and will provide new opportunities for the scientific community to use its freely available data, e.g. for the training of new machine learning models to predict sequence-structure-function relationships for diverse natural products. MIBiG 3.0 is accessible online at https://mibig.secondarymetabolites.org/
Year of Publication	2023
Journal	Nucleic Acids Research
Journal citation	51 (D1), pp. D606-D610
Digital Object Identifier (DOI)	https://doi.org/10.1093/nar/gkac1049
Open access	Published as ‘gold’ (paid) open access
Funder	Biotechnology and Biological Sciences Research Council
Funder project or code	BB/R022054/1
	BB/W013959/1
Publisher's version	gkac1049.pdf
Output status	Published
Publication dates
Online	18 Nov 2022
Publication process dates
Accepted	21 Oct 2022
Publisher	Oxford University Press (OUP)
ISSN	0305-1048

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