Boosting Geranyl Diphosphate Synthesis for Linalool Production in Engineered Yarrowia lipolytica

A - Papers appearing in refereed journals

Taratynova, M. O., Tikhonova, E. E., Fedyaeva, I. M., Dementev, D. A., Yuzbashev, T., Solovyev, A. I., Sineoky, S. P. and Yuzbasheva, E. Y. 2023. Boosting Geranyl Diphosphate Synthesis for Linalool Production in Engineered Yarrowia lipolytica. Applied Biochemistry and Biotechnology. https://doi.org/10.1007/s12010-023-04581-z

AuthorsTaratynova, M. O., Tikhonova, E. E., Fedyaeva, I. M., Dementev, D. A., Yuzbashev, T., Solovyev, A. I., Sineoky, S. P. and Yuzbasheva, E. Y.
Abstract

Linalool is a pleasant-smelling monoterpenoid widely found in the essential oils of most flowers. Due to its biologically active properties, linalool has considerable commercial potential, especially in the food and perfume industries. In this study, the oleaginous yeast Yarrowia lipolytica was successfully engineered to produce linalool de novo. The (S)-linalool synthase (LIS) gene from Actinidia argute was overexpressed to convert geranyl diphosphate (GPP) into linalool. Flux was diverted from farnesyl diphosphate (FPP) synthesis to GPP by introducing a mutated copy of the native ERG20F88W−N119W gene, and CrGPPS gene from Catharanthus roseus on its own and as part of a fusion with LIS. Disruption of native diacylglycerol kinase enzyme, DGK1, by oligo-mediated CRISPR-Cas9 inactivation further increased linalool production. The resulting strain accumulated 109.6 mg/L of linalool during cultivation in shake flasks with sucrose as a carbon source. CrGPPS expression in Yarrowia lipolytica increased linalool accumulation more efficiently than the ERG20F88W−N119W expression, suggesting that the increase in linalool production was predominantly influenced by the level of GPP precursor supply.

KeywordsLinalool; Geranyl diphosphate synthase; Yarrowia lipolytica; Monoterpenoids; Metabolic engineering
Year of Publication2023
JournalApplied Biochemistry and Biotechnology
Digital Object Identifier (DOI)https://doi.org/10.1007/s12010-023-04581-z
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online01 Jul 2023
Publication process dates
Accepted24 May 2023
PublisherHumana Press Inc
ISSN0273-2289

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