Large-scale bioproduction of natural astaxanthin in Yarrowia lipolytica

A - Papers appearing in refereed journals

Yuzbasheva, E. Y., Taratynova, M. O., Fedyaeva, I. M., Dementev, D. A., Korobov, V. S., Fedorov, A. S., Vidal, L. S., Yuzbashev, T., Sineoky, S. P. and Mikheev, M. 2023. Large-scale bioproduction of natural astaxanthin in Yarrowia lipolytica. Bioresource Technology. 21, p. 101289. https://doi.org/10.1016/j.biteb.2022.101289

AuthorsYuzbasheva, E. Y., Taratynova, M. O., Fedyaeva, I. M., Dementev, D. A., Korobov, V. S., Fedorov, A. S., Vidal, L. S., Yuzbashev, T., Sineoky, S. P. and Mikheev, M.
Abstract

Astaxanthin is a high-value chemical with strong antioxidant, anti-inflammatory and coloration properties. Here, a set of novel genetic engineering and culturing strategies for efficient astaxanthin bioproduction in Yarrowia lipolytica is reported. Modular pathway engineering and the fusion of two key enzymes (geranylgeranyl pyrophosphate synthase and bifunctional lycopene cyclase/phytoene synthase) yielded a recombinant strain with enhanced β-carotene and astaxanthin production, accumulating astaxanthin up to 131.9 mg/L in small-scale cultures with sucrose and 587.3 mg/L in a 3-L bioreactor with glucose as carbon sources, respectively. Notably, formation of intracellular carotenoid crystals was observed. Addition of oil overlayer at different regimes in 3-L bioreactors promoted secretion of astaxanthin and increased astaxanthin titer up to 973.4 g/L. Lastly, fed-batch fermentation in a 100-L bioreactor with glycerol as the carbon source resulted in a final astaxanthin titer of 812.3 mg/L after 114 h, representing the highest astaxanthin titer reported so far in large-scale cultures.

Year of Publication2023
JournalBioresource Technology
Journal citation21, p. 101289
Digital Object Identifier (DOI)https://doi.org/10.1016/j.biteb.2022.101289
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online28 Nov 2022
Publication process dates
Accepted12 Nov 2022
PublisherElsevier Sci Ltd
ISSN0960-8524

Permalink - https://repository.rothamsted.ac.uk/item/98z0y/large-scale-bioproduction-of-natural-astaxanthin-in-yarrowia-lipolytica

27 total views
0 total downloads
5 views this month
0 downloads this month