A - Papers appearing in refereed journals
Tawfike, A., Attia, E. Z., Desoukey, S. Y., Hajjar, D., Makki, A. A., Schupp, P. J., Edrada-Ebel, R. and Abdelmohsen, U. R. 2019. New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov. AMB Express. 9 (12).
|Authors||Tawfike, A., Attia, E. Z., Desoukey, S. Y., Hajjar, D., Makki, A. A., Schupp, P. J., Edrada-Ebel, R. and Abdelmohsen, U. R.|
Several approaches have been dedicated to activate the cryptic gene clusters in the genomes of actinomycetes for the targeted discovery of new fascinating biomedical lead structures. In the current study, N-acetylglucosamine was used to maximize the chemical diversity of sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. HR–ESI–MS was employed for dereplication study and orthogonal partial least square-discriminant analysis was applied to evaluate the HR–ESI–MS data of the different fractions. As a result, two new fridamycins H (1) and I (2), along with three known compounds actinosporin C (3), D (4), and G (5) were isolated from the solid culture of sponge-associated actinomycete Actinokineospora spheciospongiae sp. nov., elicited with N-acetylglucosamine. Characterization of the isolated compounds was pursued using mass spectrometry and NMR spectral data. Fridamycin H (1) exhibited significant growth inhibitory activity towards Trypanosoma brucei strain TC221. These results highlight the potential of elicitation in sponge-associated actinomycetes as an effective strategy for the discovery of new anti-infective natural products.
|Keywords||Sponges; Actinomycetes; Fridamycin; Elicitation; Elicitation; Antitrypanosomal; Actinokineospora|
|Year of Publication||2019|
|Journal citation||9 (12)|
|Digital Object Identifier (DOI)||doi:10.1186/s13568-018-0730-0|
|Open access||Published as ‘gold’ (paid) open access|
|Funder||Biotechnology and Biological Sciences Research Council|
|Online||24 Jan 2019|
|Publication process dates|
|Accepted||31 Dec 2018|
|Publisher||Biomed Central Ltd|
|Copyright license||CC BY|
Permalink - https://repository.rothamsted.ac.uk/item/8w907/new-bioactive-metabolites-from-the-elicited-marine-sponge-derived-bacterium-actinokineospora-spheciospongiae-sp-nov