Influence of Pickling Process on Allium cepa and Citrus limon Metabolome as Determined via Mass Spectrometry-Based Metabolomics

A - Papers appearing in refereed journals

Tawfike, A. F., Farag, M. A., Donia, M. S., Ehrlich, A. and Wessjohann, L. A. 2019. Influence of Pickling Process on Allium cepa and Citrus limon Metabolome as Determined via Mass Spectrometry-Based Metabolomics. Molecules. 24 (5), p. 928. https://doi.org/10.3390/molecules24050928

AuthorsTawfike, A. F., Farag, M. A., Donia, M. S., Ehrlich, A. and Wessjohann, L. A.
Abstract

Brine, the historically known food additive salt solution, has been widely used as a pickling media to preserve flavor or enhance food aroma, appearance, or other qualities. The influence of pickling, using brine, on the aroma compounds and the primary and secondary metabolite profile in onion bulb Allium cepa red cv. and lemon fruit Citrus limon was evaluated using multiplex metabolomics technologies. In lemon, pickling negatively affected its key odor compound “citral”, whereas monoterpene hydrocarbons limonene and γ-terpinene increased in the pickled product. Meanwhile, in onion sulphur rearrangement products appeared upon storage, i.e., 3,5-diethyl-1,2,4-trithiolane. Profiling of the polar secondary metabolites in lemon fruit via ultra-performance liquid chromatography coupled to MS annotated 37 metabolites including 18 flavonoids, nine coumarins, five limonoids, and two organic acids. With regard to pickling impact, notable and clear separation among specimens was observed with an orthogonal projections to least squares-discriminant analysis (OPLS-DA) score plot for the lemon fruit model showing an enrichment of limonoids and organic acids and that for fresh onion bulb showing an abundance of flavonols and saponins. In general, the pickling process appeared to negatively impact the abundance of secondary metabolites in both onion and lemon, suggesting a decrease in their food health benefits.

Keywords Allium cepa red cv; Citrus limon; Pickling; Volatiles; SPME; Chemometrics
Year of Publication2019
JournalMolecules
Journal citation24 (5), p. 928
Digital Object Identifier (DOI)https://doi.org/10.3390/molecules24050928
Web address (URL)https://www.mdpi.com/1420-3049/24/5/928
Open accessPublished as ‘gold’ (paid) open access
Publisher's version
Output statusPublished
Publication dates
Online07 Mar 2019
Publication process dates
Accepted17 Feb 2019
PublisherMDPI
Copyright licenseCC BY
ISSN1420-3049

Permalink - https://repository.rothamsted.ac.uk/item/8wq03/influence-of-pickling-process-on-allium-cepa-and-citrus-limon-metabolome-as-determined-via-mass-spectrometry-based-metabolomics

36 total views
73 total downloads
2 views this month
3 downloads this month
Download files as zip