Genome assembly and transcriptomic analysis to elucidate the ability of Nasonovia ribisnigri to break host plant resistance

A - Papers appearing in refereed journals

Garrett, D., Teakle, G., Collier, R., Bell, J., Cerezo-Medina, S. and Morales-Hojas, R. 2024. Genome assembly and transcriptomic analysis to elucidate the ability of Nasonovia ribisnigri to break host plant resistance. Insect Molecular Biology. pp. 1-18. https://doi.org/10.1111/imb.12894

AuthorsGarrett, D., Teakle, G., Collier, R., Bell, J., Cerezo-Medina, S. and Morales-Hojas, R.
Abstract

Aphid genomic resources enable the study of complex life history traits and provide information on vector biology, host adaption and speciation. The currant-lettuce aphid (Nasonovia ribisnigri) is a cosmopolitan pest of outdoor lettuce (Lactuca sativa). Until recently, the use of resistant cultivars was an effective method for managing N. ribisnigri. A resistant cultivar containing a single gene (Nr-locus), introduced in the 1980’s, conferred complete resistance to feeding. Overreliance of this Nr-locus in lettuce resulted in N. ribisnigri ability to break resistance mechanism, with first reports during 2003. Our work attempts to understand which candidate gene(s) are associated with this resistance breaking mechanism.

We present two de novo draft assembles for N. ribisnigri genomes, corresponding to both avirulent (Nr-locus susceptible) and virulent (Nr-locus resistant) biotypes. Changes in gene expression of the two N. ribisnigri biotypes were investigated using transcriptomic analyses of RNA-seq data to understand the potential mechanisms of resistance to the Nr-locus in lettuce. The draft genome assemblies were 94.2% and 91.4% complete for the avirulent and virulent biotypes, respectively. Out of the 18,872 differentially expressed genes, a single gene/locus was identified in N. ribisnigri that was shared between two resistant-breaking biotypes. This locus was further explored and validated in qRT-PCR experiments and has predicted localisations in both the cytoplasm and nucleus. This is the first study to provide evidence that a single gene/locus is likely responsible for the ability of N. ribisnigri to overcome the Nr-locus resistance in the lettuce host.

KeywordsResistance-breaking mechanisms; Insect pest; Host plant resistance; Gene for gene resistance; Currant-lettuce aphid
Year of Publication2024
JournalInsect Molecular Biology
Journal citationpp. 1-18
Digital Object Identifier (DOI)https://doi.org/10.1111/imb.12894
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeThe Rothamsted Insect Survey - National Capability [2017-2022]
Publisher's version
Accepted author manuscript
Supplemental file
Output statusPublished
Publication dates
Online13 Feb 2024
Publication process dates
Accepted09 Jan 2024
PublisherWiley
ISSN0962-1075

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